Rapid semen identification from mixed body fluids using methylation-sensitive high-resolution melting analysis of the DACT1 gene
| Autor: | Osamu Ogawa, Eriko Hirai, Yuya Hamano, Keiji Tamaki, Shuntaro Fujimoto, Kentaro Ichioka, Sho Manabe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Forensic Genetics
Male endocrine system Saliva Semen 01 natural sciences High Resolution Melt Pathology and Forensic Medicine Andrology 03 medical and health sciences chemistry.chemical_compound fluids and secretions 0302 clinical medicine Humans 030216 legal & forensic medicine Gene Crime Victims Adaptor Proteins Signal Transducing Body fluid urogenital system 010401 analytical chemistry Nuclear Proteins DNA DNA Methylation Spermatozoa Sperm Body Fluids 0104 chemical sciences Issues ethics and legal aspects chemistry DNA methylation Female Crime |
| Zdroj: | Legal Medicine. 48:101806 |
| ISSN: | 1344-6223 |
| DOI: | 10.1016/j.legalmed.2020.101806 |
| Popis: | In forensic genetics, a suspect is assigned to a component of a DNA mixture profile, and a probabilistic interpretation is then usually performed. However, it is difficult to determine what types of body fluid the component is from. Previous studies have reported that the fourth exon of the Dishevelled binding antagonist of beta catenin 1 (DACT1) gene is hypomethylated in a semen DNA-specific manner. In the present study, we evaluated whether the DACT1 gene could be effectively used to identify semen in body fluid mixtures and were able to semi-quantify the semen DNA content in mixed fluids. Our results showed that the DACT1 gene was useful in discriminating semen from venous blood and saliva. However, the amount of sperm in semen can affect semen identification. In addition, SI (the semen DNA content index), which we developed, was useful to determine whether the semen compromised majority, almost half, or was in the minority of the components in a mixed fluid. This technique is based on the methylation-sensitive high-resolution melting (MS-HRM) technology, which is time-, cost-, and labour-effective, and could be adopted in routine criminal investigations. |
| Databáze: | OpenAIRE |
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