Differences in energy metabolism and neuromuscular transmission between 30-Hz and 100-Hz stimulation in rat skeletal muscle
| Autor: | Takaaki Ikata, Shinjiro Takata |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Intracellular Fluid
Male medicine.medical_specialty Magnetic Resonance Spectroscopy Intracellular pH Physical Exertion Neuromuscular Junction Neuromuscular transmission Physical Therapy Sports Therapy and Rehabilitation Stimulation Electromyography Hindlimb Rats Inbred WKY Synaptic Transmission Internal medicine medicine Animals Muscle Skeletal medicine.diagnostic_test business.industry Rehabilitation Phosphorus Isotopes Skeletal muscle Hydrogen-Ion Concentration Sciatic Nerve Electric Stimulation Rats medicine.anatomical_structure Endocrinology Sciatic nerve medicine.symptom Energy Metabolism business Muscle Contraction Muscle contraction |
| Zdroj: | Archives of Physical Medicine and Rehabilitation. 82:666-670 |
| ISSN: | 0003-9993 |
| DOI: | 10.1053/apmr.2001.21942 |
| Popis: | To examine differences in energy metabolism and neuromuscular transmission failure in rat hindlimb muscles subjected to electric stimulation at different frequencies.Experimental animal study.Bioenergetic Research Center at Otsuka Pharmaceutical Company, Otsuka, Japan.Thirty-two 25-week-old male Wistar-Kyoto rats.With the rat under general anesthesia, the sciatic nerve was electrically stimulated at 30Hz and 100Hz to induce muscle contraction.Energy level and intracellular pH of muscles by phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS); M-wave amplitude of muscles by electromyography.During the first 4 minutes under stimulation at 30Hz and at 100Hz, energy level and intracellular pH dropped to their lowest values (p.05 or p.01); the values then recovered with time. Recovery rates of energy level and intracellular pH during stimulation at 100Hz were greater than those observed during stimulation at 30Hz. The M-wave amplitude during 100-Hz stimulation was permanently and significantly lower than that measured during 30-Hz stimulation (p.01), and the recovery rate of M-wave amplitude after stimulation at 100Hz was slower than that after stimulation at 30Hz.Neuromuscular transmission failure was greater with 100-Hz stimulation than with 30-Hz stimulation. This finding may account for the rapid recovery of energy level and intracellular pH that occurs with stimulation at 100Hz. |
| Databáze: | OpenAIRE |
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