Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection
| Autor: | Phani Rama Krishna Behra, B. M. Fredrik Pettersson, Leif A. Kirsebom, Don G. Ennis, Kate M. Root, Amrita Mallick, Sarbashis Das, Santanu Dasgupta, Martin Cheramie, Pamela L. C. Small, Malavika Ramesh, Heather R. Jordan, Irma de la Cruz Melara |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
animal structures
Transcription Genetic lcsh:Medicine Sigma Factor Bacterial genetics Microbiology Mycobacterium tuberculosis Bacterial Proteins Species Specificity Sigma factor Stress Physiological Gene expression Animals RNA Messenger alpha-Crystallins Heat shock lcsh:Science Gene Mycobacterium marinum Regulation of gene expression Multidisciplinary biology lcsh:R fungi Gene Expression Regulation Bacterial biology.organism_classification 3. Good health Culicidae Larva bacteria lcsh:Q Heat-Shock Response Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0139823 (2015) |
| ISSN: | 1932-6203 |
| Popis: | We have used RNASeq and qRT-PCR to study mRNA levels for all σ-factors in different Mycobacterium marinum strains under various growth and stress conditions. We also studied their levels in M. marinum from infected fish and mosquito larvae. The annotated σ-factors were expressed and transcripts varied in relation to growth and stress conditions. Some were highly abundant such as sigA, sigB, sigC, sigD, sigE and sigH while others were not. The σ-factor mRNA profiles were similar after heat stress, during infection of fish and mosquito larvae. The similarity also applies to some of the known heat shock genes such as the α-crystallin gene. Therefore, it seems probable that the physiological state of M. marinum is similar when exposed to these different conditions. Moreover, the mosquito larvae data suggest that this is the state that the fish encounter when infected, at least with respect to σ-factor mRNA levels. Comparative genomic analysis of σ-factor gene localizations in three M. marinum strains and Mycobacterium tuberculosis H37Rv revealed chromosomal rearrangements that changed the localization of especially sigA, sigB, sigD, sigE, sigF and sigJ after the divergence of these two species. This may explain the variation in species-specific expression upon exposure to different growth conditions. |
| Databáze: | OpenAIRE |
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