Nuclear Import Defect of Human Immunodeficiency Virus Type 1 DNA Flap Mutants Is Not Dependent on the Viral Strain or Target Cell Type
| Autor: | Pierre Charneau, Sandie Munier, Philippe Souque, Nathalie J. Arhel, Karine Mollier |
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| Přispěvatelé: | Virologie moléculaire et Vectorologie, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by grants from the Institut Pasteur, the Agence Nationale de Recherche sur le SIDA (ANRS), the Agence Nationale de la Recherche (ANR), and the Fondation pour la Recherche Médicale (FRM)., Wild-type and cPPT mutant provirus clones were provided by M. H. Malim (YU-2 and LAI) and A. Engelman (NL4-3 and HXB2)., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
[SDV]Life Sciences [q-bio]
viruses Immunology Mutant Active Transport Cell Nucleus Context (language use) Biology medicine.disease_cause Microbiology Virus 03 medical and health sciences chemistry.chemical_compound Virology medicine Humans 030304 developmental biology Infectivity 0303 health sciences Mutation 030302 biochemistry & molecular biology virus diseases Provirus beta-Galactosidase 3. Good health Virus-Cell Interactions chemistry Insect Science [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology DNA Viral HIV-1 Viral disease DNA HeLa Cells |
| Zdroj: | Journal of Virology Journal of Virology, American Society for Microbiology, 2006, 80 (20), pp.10262-10269. ⟨10.1128/JVI.00974-06⟩ Journal of Virology, 2006, 80 (20), pp.10262-10269. ⟨10.1128/JVI.00974-06⟩ |
| ISSN: | 0022-538X 1098-5514 |
| Popis: | We have previously established, using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear import. Although the impact of the DNA Flap on nuclear import has already found numerous independent confirmations in the context of lentivirus vectors, it has been claimed that it may be nonessential for infectious virus strains LAI, YU-2 (J. D. Dvorin et al., J. Virol. 76: 12087-12096, 2002), HXB2, and NL4-3 (A. Limon et al., J. Virol. 76: 12078-12086, 2002). We conducted a detailed analysis of virus infectivity using the provirus clones provided by the authors and analogous target cells. In contrast to published data, our results show that all cPPT mutant viruses exhibit reduced infectivity corresponding to a nuclear import defect irrespective of the viral genetic background or target cell. |
| Databáze: | OpenAIRE |
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