Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal differentiation
| Autor: | Katsuhiko Arai, Takashi Matsumoto, Kazuhiko Arahara, Fumiki Morimatsu |
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| Rok vydání: | 2015 |
| Předmět: |
animal structures
Neurite integrin Cellular differentiation Integrin Tretinoin Embryoid body Biology Biochemistry Shh Mice fibronectin Laminin Cell Line Tumor retinoic acid Animals Hedgehog Proteins RNA Messenger Sonic hedgehog neuronal differentiation Neurons General Veterinary Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Note Molecular biology Fibronectins Up-Regulation Fibronectin P19 cell Gene Expression Regulation embryonic structures biology.protein |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.15-0122 |
| Popis: | In this study, the effect of fibronectin on the neurite outgrowth from embryoid bodies (EBs) in neurodifferentiated embryonal carcinoma P19 cells was examined. The neurite outgrowth on fibronectin was maintained for a longer time in comparison with those on collagen or laminin. Quantitative RT-PCR revealed that mRNA level corresponding to sonic hedgehog (Shh) in neurodifferentiated P19 cells was upregulated on fibronectin, whereas collagen or laminin did not affect. Further knockdown of integrin αv subunit in P19 cells demonstrated that expression of Shh was mediated through interaction between fibronectin and integrin. Additionally, exogenous Shh agonist accelerated neurite outgrowth from embryonic stem cell-derived EBs without large change of neuronal phenotype expression. Taken together, fibronectin could maintain neurite outgrowth via increased Shh expression. |
| Databáze: | OpenAIRE |
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