Requirement of CD80 and CD86 Molecules for Antigen Presentation by Eosinophils
| Autor: | Norihisa Ishii, Masako Nagoya, Teruaki Amano, Mutsuhiko Minami, H. Nakazima, N. Tamura, Masahiro Yoshinari, Masatoshi Nakazawa |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Ovalbumin
medicine.drug_class T-Lymphocytes Immunology Antigen presentation Mice Transgenic chemical and pharmacologic phenomena Thymus Gland Major histocompatibility complex Monoclonal antibody Enterotoxins Mice Antigen Antigens CD medicine Animals Cytotoxic T cell Lymph node CD86 Antigen Presentation Mice Inbred C3H Membrane Glycoproteins Superantigens biology Histocompatibility Antigens Class II hemic and immune systems General Medicine respiratory system Eosinophils medicine.anatomical_structure B7-1 Antigen biology.protein Female B7-2 Antigen Lymph Nodes Interleukin-5 Cell Division CD80 |
| Zdroj: | Scandinavian Journal of Immunology. 44:229-238 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1046/j.1365-3083.1996.d01-303.x |
| Popis: | The authors analysed the antigen-presenting ability of eosinophils purified from peritoneal exudate cells of interleukin-5 (IL-5) transgenic mice. The granulocyte-macrophage colony-stimulating factor (GM-CSF)-treated eosinophils induced proliferative responses of primed lymph node T cells and thymus T cells to staphylococcal enterotoxin B (SEB), while untreated eosinophils induced little or no response. GM-CSF-treated eosinophils also induced proliferation of ovalbumin (OVA)-primed lymph node T cells to OVA. Although untreated eosinophils expressed no MHC class II molecule on the surface the eosinophils could be induced to express major histocompatibility complex (MHC) class II molecules when treated with GM-CSF. In the present study, anti-I-Ak monoclonal antibodies (MoAbs) strongly inhibited proliferation of thymus T cells and proliferation of OVA-primed lymph node T cells in response to OVA, but weakly inhibited proliferation of primed T cells in response to SEB. Furthermore, CD80 (B7-1) and CD86 (B7-2) were expressed on the surfaces of untreated eosinophils. The expression of those two molecules on the eosinophils was increased by incubation with GM-CSF. Moreover, anti-CD80 or anti-CD86 MoAbs blocked proliferative responses of primed lymph node T cells and thymus T cells to SEB, and also blocked responses of primed lymph node T cells to OVA. Thus, CD80 and CD86 play an important role in stimulation of T cells by eosinophils. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text