Polymorphism of CSN1S1 (g.12164G>A) and CSN2 (g.8913C>A) genes in pure and cross dairy goats
| Autor: | Cece Sumantri, Oktiq Kurnia Sari, Lailatus Syifa, Anneke Anggraeni, Andi Baso Lompengeng Ishak |
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| Rok vydání: | 2021 |
| Předmět: |
dairy goats
Environmental Engineering Physiology Locus (genetics) Biology Microbiology QR1-502 Industrial and Manufacturing Engineering Genotype frequency Loss of heterozygosity Animal science QL1-991 Polymorphism (computer science) Casein csn1s1 gene genetic polymorphism QP1-981 Allele csn2 gene Zoology Gene Allele frequency |
| Zdroj: | BIO Web of Conferences, Vol 33, p 02001 (2021) |
| ISSN: | 2117-4458 |
| DOI: | 10.1051/bioconf/20213302001 |
| Popis: | Casein genes directly control milk protein of animals. CSN1S1 (αS1-Casein) and CSN2 (β-Casein) genes influence on milk protein fractions. Genetic polymorphisms of CSN1S1 gene at g.12164G>A locus and CSN2 gene at g.8913C>A locus were identified by PCR-RFLP technique. Animal samples were pure dairy goats providing PE (5 hds.), Saanen (8 hds.) and their crosses providing Sapera (50% Saanen, 50% PE) (51 hds.) and SaanPE (75% Saanen, 25% PE) (3 hds.) from IRIAP dairy goat station. Allele frequency, genotype frequency, heterozygosity value, and Hardy-Weinberg (H-W) equilibrium value were analyzed by Popgen32 program. CSN1S1_g.12164G>A locus resulted in two alleles, i.e. G allele (192 bp, 145 bp, and 101 bp) and A allele (337 bp and 101 bp). The G allele from the highest frequenciest was successively Saanen (0.625), Sapera (0.578), PE (0.400), and SaanPE (0.333). Most dairy goats were heterozygote (Ho>He) and in H-W equilibrium (q2 count < q2P0.05). Whereas CSN2_g.8913C>A locus was monomorphic for possesing only C allele (233 bp and 162 bp), without A allele (416 bp). The existent g.12164G>A SNP of the CSN1S1 gene of could be a potencial molecular selection marker of milk protein content in dairy goat. |
| Databáze: | OpenAIRE |
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