Copper Transport Across Pea Thylakoid Membranes
| Autor: | Richard Shingles, Richard E. McCarty, Larry E. Wimmers |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
inorganic chemicals
Time Factors Physiology Inorganic chemistry chemistry.chemical_element Plant Science Zinc Thylakoids Fluorescence Pisum Cations Genetics Chelation Organic Chemicals Chelating Agents Fluorescent Dyes Manganese biology Peas food and beverages Biological Transport biology.organism_classification Copper Chloroplast Membrane chemistry Thylakoid Biophysics Research Article |
| Zdroj: | Plant Physiology. 135:145-151 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.103.037895 |
| Popis: | The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu2+-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration and saturated at about 0.6 μ m total Cu2+. Cu2+ uptake was maximal at a thylakoid lumen pH of 7.0. Cu2+ transport was inhibited by Zn2+ but was largely unaffected by Mn2+ and Cu+. Zn2+ inhibited Cu2+ transport to a maximum of 60%, indicating that there may be more than one transporter for copper in pea thylakoid membranes. |
| Databáze: | OpenAIRE |
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