In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection
| Autor: | Roman Pantůček, Radek Novotný, Anthony V Florschütz, Jiri Gallo, Milan Kolar, Michaela Kesselová |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Prosthesis-Related Infections
medicine.drug_class Joint Prosthesis Staphylococcus Antibiotics macromolecular substances Microbial Sensitivity Tests Bacterial growth In Vitro Techniques medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Vancomycin medicine Humans Polymethyl Methacrylate Arthroplasty Replacement business.industry technology industry and agriculture Bone Cements equipment and supplies Bone cement In vitro Anti-Bacterial Agents Staphylococcus aureus Biofilms Gentamicin Gentamicins Polyethylenes business Biomedical engineering medicine.drug |
| Zdroj: | Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia. 149(1) |
| ISSN: | 1213-8118 |
| Popis: | Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent perioperative bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro effects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh molecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identified using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantification. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth. Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement. |
| Databáze: | OpenAIRE |
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