Identification of a stress-induced factor of Corynebacterineae that is involved in the regulation of the outer membrane lipid composition
| Autor: | Cécile Labarre, Xavier Meniche, Marielle Tropis, Emilie Huc, Nicolas Bayan, Célia de Sousa-d'Auria, Françoise Laval, Damien Portevin, Christine Houssin, Mamadou Daffé, Christophe Guilhot |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Mycobacterium tuberculosis
Protein family Membrane lipids Mycobacterium smegmatis Biology Microbiology Corynebacterium glutamicum Mycolic acid 03 medical and health sciences Membrane Lipids Bacterial Proteins MESH: Reverse Transcriptase Polymerase Chain Reaction Corynebacterineae [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene Regulation MESH: Bacterial Proteins Molecular Biology MESH: Mycobacterium smegmatis MESH: Lipid Metabolism 030304 developmental biology 2. Zero hunger Regulation of gene expression chemistry.chemical_classification MESH: Gene Expression Regulation Bacterial 0303 health sciences 030306 microbiology Reverse Transcriptase Polymerase Chain Reaction Temperature MESH: Corynebacterium glutamicum Lipid metabolism Gene Expression Regulation Bacterial Mycobacterium tuberculosis biology.organism_classification Lipid Metabolism MESH: Temperature Biochemistry chemistry MESH: Membrane Lipids Bacterial outer membrane |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2009, 191 (23), pp.7323-32. ⟨10.1128/JB.01042-09⟩ |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/JB.01042-09⟩ |
| Popis: | Corynebacterineae are gram-positive bacteria that possess a true outer membrane composed of mycolic acids and other lipids. Little is known concerning the modulation of mycolic acid composition and content in response to changes in the bacterial environment, especially temperature variations. To address this question, we investigated the function of the Rv3802c gene, a gene conserved in Corynebacterineae and located within a gene cluster involved in mycolic acid biosynthesis. We showed that the Rv3802 ortholog is essential in Mycobacterium smegmatis , while its Corynebacterium glutamicum ortholog, NCgl2775, is not. We provided evidence that the NCgl2775 gene is transcriptionally induced under heat stress conditions, and while the corresponding protein has no detectable activity under normal growth conditions, the increase in its expression triggers an increase in mycolic acid biosynthesis concomitant with a decrease in phospholipid content. We demonstrated that these lipid modifications are part of a larger outer membrane remodeling that occurs in response to exposure to a moderately elevated temperature (42°C). In addition to showing an increase in the ratio of saturated corynomycolates to unsaturated corynomycolates, our results strongly suggested that the balance between mycolic acids and phospholipids is modified inside the outer membrane following a heat challenge. Furthermore, we showed that these lipid modifications help the bacteria to protect against heat damage. The NCgl2775 protein and its orthologs thus appear to be a protein family that plays a role in the regulation of the outer membrane lipid composition of Corynebacterineae under stress conditions. We therefore propose to name this protein family the envelope lipids regulation factor (ElrF) family. |
| Databáze: | OpenAIRE |
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