Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques
| Autor: | Carla A.M. Portugal, Jan C.T. Eijkel, Roald M. Tiggelaar, Reyes Mallada, Mariella Polino, Maria Pilar Pina, Isabel M. Coelhoso, João G. Crespo |
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| Přispěvatelé: | Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), European Commission, Physics of Fluids, Mesoscale Chemical Systems, Biomedical and Environmental Sensorsystems |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Work (thermodynamics)
Materials science 010405 organic chemistry Nucleation 22/2 OA procedure Nafion membrane Crystal growth General Chemistry 010402 general chemistry Condensed Matter Physics 01 natural sciences 0104 chemical sciences Membrane Chemical engineering General Materials Science Protein crystallization Nanoscopic scale Lithography |
| Zdroj: | Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Digital.CSIC. Repositorio Institucional del CSIC Crystal growth & design, 20(4), 2174-2186. American Chemical Society |
| ISSN: | 1528-7483 |
| Popis: | In this work, the influence of surface topography on protein crystallization over Nafion is investigated. Two types of Nafion-based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From an analysis of the induction time, nucleation, and crystal growth rate of trypsin from bovine pancreas, all of the patterned Nafion-based membranes show an enhanced nucleation and crystal growth. To provide additional insight into the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both the structural and chemical properties of the nucleant Nafion surface. This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from the FCT/MCTES (UID/QUI/50006/2019). M.P. thanks the Education, Audiovisual and Culture Executive Agency (EACEA) for a Ph.D. grant under the Program “Erasmus Mundus Doctorate in Membrane Engineering”-EUDIME (FPA 2011-2014, http://www.eudime.unical.it). |
| Databáze: | OpenAIRE |
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