miR‑125b/NRF2/HO‑1 axis is involved in protection against oxidative stress of cystic fibrosis: A pilot study
| Autor: | Maria Pelullo, Isabella Screpanti, Samantha Cialfi, Daniela Savi, Serena Quattrucci, Antonio Pizzuti, Claudio Talora, Giuseppe Cimino |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research Inflammation medicine.disease_cause Cystic fibrosis cystic fibrosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) Downregulation and upregulation microRNA medicine oxidative stress Oncogene biology cystic fibrosis transmembrane conductance regulator Articles General Medicine Glutathione microRNA‑125b medicine.disease Cystic fibrosis transmembrane conductance regulator heme oxygenase‑1 030104 developmental biology chemistry 030220 oncology & carcinogenesis Cancer research biology.protein medicine.symptom Oxidative stress |
| Zdroj: | Exp Ther Med |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | In the physiopathology of cystic fibrosis (CF), oxidative stress implications are recognized and widely accepted. The cystic fibrosis transmembrane conductance regulator (CFTR) defects disrupt the intracellular redox balance causing CF pathological hallmarks. Therefore, oxidative stress together with aberrant expression levels of detoxification genes and microRNAs (miRNAs/miRs) may be associated with clinical outcome. Using total RNA extracted from epithelial nasal cells, the present study analyzed the expression levels of oxidative stress genes and one miRNA using quantitative PCR in a representative number of patients with CF compared with in healthy individuals. The present pilot study revealed the existence of an association among CFTR, genes involved in the oxidative stress response and miR-125b. The observed downregulation of CFTR gene expression was accompanied by increased expression levels of Nuclear factor erythroid derived-2 like2 and its targets NAD(P)H:Quinone Oxidoreductase and glutathione S-transferase 1. Moreover, the expression levels of heme oxygenase-1 (HO-1) and miR-125b were positively correlated with a forced expiratory volume in 1 sec (FEV1) >60% in patients with CF with chronic Pseudomonas aeruginosa lung infection (r=0.74; P |
| Databáze: | OpenAIRE |
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