Human peripheral blood CD8+ CD28- T cells of renal allograft recipients do not express FOXP3 protein
| Autor: | Andrzej Górski, Grażyna Korczak-Kowalska, Mariusz Niemczyk, A Duszota, D Kłosowska, P. Wierzbicki, Anna Korecka-Polak, Katarzyna Bocian, Leszek Paczek |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Graft Rejection Male medicine.medical_treatment Population chemical and pharmacologic phenomena Biology CD8-Positive T-Lymphocytes Peripheral blood mononuclear cell Flow cytometry CD28 Antigens T-Lymphocyte Subsets medicine Humans education Incubation Aged Sirolimus Transplantation Messenger RNA education.field_of_study medicine.diagnostic_test FOXP3 hemic and immune systems Immunosuppression Forkhead Transcription Factors Middle Aged Molecular biology Kidney Transplantation Case-Control Studies Immunology Chronic Disease Cyclosporine Surgery Female CD8 Biomarkers Immunosuppressive Agents |
| Zdroj: | Transplantation proceedings. 43(8) |
| ISSN: | 1873-2623 |
| Popis: | Introduction In recent studies, the FOXP3 molecule has been suggested to be a marker of a suppressor subset of human CD8+ CD28− T cells based on correlations between the level of its mRNA and allograft function. Because this transcriptional factor produces a protein, we suggest that these correlations should focus on the FOXP3 protein. The aim of our study was to evaluate whether FOXP3 protein was present in cells of the CD8+ CD28− population in the peripheral blood of renal allograft recipients and whether the level of CD8+ CD28− FOXP3+ cells correlated with allograft function. Methods The study was performed on 30 renal allograft recipients with uneventful stable courses (n = 18) or biopsy-proven chronic rejection (n = 12). The immunosuppression was based on cyclosporine (n = 12) or rapamycin (n = 9). Peripheral blood mononuclear cells isolated from recipient blood samples were labeled with anti-CD8 and anti-CD28 MAbs conjugated with fluorochromes. After incubation, washing, and labeling using a PE anti-human FOXP3 Kit, we determined the percentage of cells by flow cytometry. Results FOXP3 protein expression was not observed either in the CD8+ CD28− population, or the whole populations of CD8+ or CD28− cells among patient groups. Conclusions The expression of FOXP3 protein in CD8+ CD28− cells seems to be of a questionable value as a diagnostic tool for allograft function, it is probably not a marker for the CD8+ CD28− T cell subset. |
| Databáze: | OpenAIRE |
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