Antitumor Activity of New Olivacine Derivatives
| Autor: | Benita Wiatrak, Agnieszka Dobosz, Beata Tylińska, Tomasz Gębarowski, Janusz Piasny |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Aspidosperma
Cell Survival Pharmaceutical Science Antineoplastic Agents Apoptosis Olivacine Pharmacology P-glycoprotein Rhodamine 123 Article Analytical Chemistry Cell Line lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Cell Line Tumor Drug Discovery Humans MTT assay Viability assay ATP Binding Cassette Transporter Subfamily B Member 1 Ellipticines Physical and Theoretical Chemistry 030304 developmental biology Cell Proliferation 0303 health sciences biology Chemistry olivacine Organic Chemistry pyridocarbazole Chemistry (miscellaneous) Cell culture Drug Resistance Neoplasm 030220 oncology & carcinogenesis biology.protein Molecular Medicine Efflux Drug Screening Assays Antitumor multiple drug resistance |
| Zdroj: | Molecules Volume 25 Issue 11 Molecules, Vol 25, Iss 2512, p 2512 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25112512 |
| Popis: | Olivacine is an alkaloid-containing pyridocarbazole structure. It is isolated from the bark of the evergreen timber tree, Aspidosperma olivaceum. Its well-documented anticancer activity led to the synthesis of new derivatives, which are semisynthetic and fully synthetic pyridocarbazoles. This study aimed to evaluate the potential antineoplastic activity of four newly synthesized olivacine derivatives. Multidrug resistance is a common phenomenon causing failure in the chemotherapy of many tumors. It is mainly related to increased function of P-glycoprotein, an efflux pump removing cytostatic out of the cells. The cell lines used in the study were colorectal carcinoma cell lines: LoVo (doxorubicin-sensitive) and LoVo/DX (doxorubicin-resistant). The NHDF cell line was used to assess cell viability. First, the cells were incubated with olivacine derivatives. In the next step, the following assays were performed: DCF-DA assay, MTT assay, rhodamine 123 assay, detection of apoptosis, proliferation inhibition-mitotic index. The tested compounds showed higher antineoplastic potential and lower toxicity than the reference compound ellipticine. The results indicate that the new olivacine derivatives are good candidates for future anticancer drugs. |
| Databáze: | OpenAIRE |
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