Structural elucidation of galactomannan from seeds of Crotalaria�mucronata Desv. by atomic force microscopy
| Autor: | Shou‑Jun Guo, Li‑Ling Xie, Yong‑Li Yang, Yu‑Zhong Zheng, Yong Zhang, Zheng‑Xia Zhang, Ang Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Cancer Research
Static Electricity Microscopy Atomic Force Polysaccharide 01 natural sciences Biochemistry Endosperm Mannans symbols.namesake Galactomannan chemistry.chemical_compound Carbohydrate Conformation Genetics Molecular Biology Protein secondary structure chemistry.chemical_classification biology 010405 organic chemistry Chemistry Hydrogen bond Crotalaria Galactose Hydrogen Bonding biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Crystallography Oncology Seeds Helix symbols Molecular Medicine van der Waals force Mannose |
| Zdroj: | Molecular Medicine Reports. |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.8280 |
| Popis: | A non-ionic water-soluble galactomannan, isolated from the seed endosperm of Crotalaria mucronata Desv., may be an ideal thickener to increase food viscosity. The present study hypothesized that the viscous property of Crotalaria galactomannan may be associated with its spatial structure. Therefore, the structure of Crotalaria galactomannan was elucidated using an atomic force microscope. The results of the present study demonstrated that the polysaccharide consisted of a D‑mannose backbone with D‑galactose branches, and the D‑mannose/D‑galactose ratio was 2.375:1. In the three‑dimensional structure of Crotalaria galactomannan, the helix was a common secondary structure, containing numerous ring structures of different sizes. In addition, multiple helixes may link together via hydrogen bonding and van der Waals forces, forming aggregations with small rings or spiral windings. The results of the present study indicated that the multiple‑branching construction of Crotalaria galactomannan may underlie its viscosity‑enhancing properties in the water phase. |
| Databáze: | OpenAIRE |
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