Multiphoton Microscopy for Caries Detection with ICDAS Classification
| Autor: | Delphine Tardivo, Hervé Tassery, Ivan Panayotov, Bernard Levallois, Amel Slimani, Thierry Cloitre, Csilla Gergely, Elodie Terrer, Frédéric Cuisinier |
|---|---|
| Přispěvatelé: | Laboratoire de Bioingénierie et NanoSciences (LBN), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Aix-Marseille Université - Faculté d'odontologie (AMU ODONTO), Aix Marseille Université (AMU), Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Materials science [SDV]Life Sciences [q-bio] [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Dental Caries 01 natural sciences 010309 optics 03 medical and health sciences Nonlinear optical 0302 clinical medicine stomatognathic system 0103 physical sciences Dentin medicine Humans Organic matrix Dental Enamel General Dentistry Histological examination Collagen type Enamel paint 030206 dentistry stomatognathic diseases medicine.anatomical_structure Multiphoton fluorescence microscope Microscopy Fluorescence Multiphoton visual_art visual_art.visual_art_medium Disease Progression Carious lesion Biomedical engineering |
| Zdroj: | Caries Research Caries Research, Karger, 2018, ⟨10.1159/000486428⟩ Caries Research, 2018, 52 (5), pp.359-366. ⟨10.1159/000486428⟩ |
| ISSN: | 0008-6568 1421-976X |
| Popis: | International audience; Dentin carious lesion is a dynamic process that involves de- mineralization and collagen denaturation. Collagen type I is the major protein in dentin and it has been investigated based on its optical properties. Multiphoton microscopy (MPM) is a nonlinear imaging technique that reveals the car- ies process using the collagen two-photon excitation fluo- rescence (2PEF) and its second-harmonic generation (SHG). Combining the histological and the International Caries De- tection and Assessment System (ICDAS) classifications with nonlinear optical spectroscopy (NLOS), 2PEF and SHG inten- sities of enamel and dentin were highly altered during the caries process. It has been proven that the ratio SHG/2PEF is a relevant indicator of the organic matrix denaturation [Ter- rer et al.: J Dent Res 2016;96:574–579]. In the present study, a series of measurable signals is made to detect early stages of carious lesion according to the ICDAS classification and to explore the relationship between these measures and theICDAS scale. Comparison of the efficiency of nonlinear opti- cal signals for caries detection with the ICDAS classification is essential to evaluate their potential for clinical applica- tion. In our study, the use of the NLOS measured by MPM allowed us to monitor a quantitative parameter (SHG/2PEF ratio) according to the dentin carious lesion state (ICDAS and histological examination). Three coherent new groups were defined (ICDAS 0/1; ICDAS 2/3; ICDAS 4/5/6), where the carious process can be clearly described with a statistically significant decrease of the SHG/2PEF ratio. |
| Databáze: | OpenAIRE |
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