Streptococcus caviae sp. nov., isolated from guinea pig faecal samples
| Autor: | Caroline M. Plugge, Willem M. de Vos, John van der Oost, Loes J Hilderink, Susakul Palakawong Na Ayudthaya |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Cellobiose Guinea Pigs Cavia medicine.disease_cause Microbiology Guinea pig Feces 03 medical and health sciences chemistry.chemical_compound Microbiologie RNA Ribosomal 16S medicine Animals Raffinose Phylogeny Ecology Evolution Behavior and Systematics VLAG 2. Zero hunger WIMEK biology Strain (chemistry) Streptococcus Fatty Acids Inulin Nucleic Acid Hybridization food and beverages Lactic acid Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology chemistry Fermentation Dried grass Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology 67 (2017) 5 International Journal of Systematic and Evolutionary Microbiology, 67(5), 1551-1556 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001758 |
| Popis: | A novel cellobiose-degrading and lactate-producing bacterium, strain Cavy grass 6T, was isolated from faecal samples of guinea pigs (Cavia porcellus). Cells of the strain were ovalshaped, non-motile, non-spore-forming, Gram-stain-positive and facultatively anaerobic. The strain gr at 25–40 °C (optimum 37 °C) and pH 4.5–9.5 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cavy grass 6T belongs to the genus Streptococcus with its closest relative being Streptococcus devriesei CCUG 47155T with only 96.5 % similarity. Comparing strain Cavy grass 6T and Streptococcus devriesei CCUG 47155T, average nucleotide identity and level of digital DNA–DNA hybridization dDDH were only 86.9 and 33.3 %, respectively. Housekeeping genes groEL and gyrA were different between strain Cavy grass 6T and other streptococci. The G+C content of strain Cavy grass 6T was 42.6±0.3 mol%. The major (>10 %) cellular fatty acids of strain Cavy grass 6T were C16:0, C20 : 1ω9c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). Strain Cavy grass 6T ferment a range of plant mono- and disaccharides as well as polymeric carbohydrates, including cellobiose, dulcitol, d-glucose, maltose, raffinose, sucrose, l-sorbose, trehalose, inulin and dried grass extract, to lactate, formate, acetate and ethanol. Based on phylogenetic and physiological characteristics, Cavy grass 6T can be distinguished from other members of the genus Streptococcus . Therefore, a novel species of the genus Streptococcus , family Streptococcaceae , order Lactobacillales is proposed, Streptococcus caviae sp. nov. (type strain Cavy grass 6T=TISTR 2371T=DSM 102819T). |
| Databáze: | OpenAIRE |
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