Surgical acquired aganglionosis: myth or reality?
| Autor: | S. Salas, D. Meléndez, Y. Quiroga, Sandra Montedonico, F. Villagrán, Tamara Sáez, M. J. Bag, H. Vallejos, J. Varas |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Ischemia Anastomosis Gastroenterology Enteric Nervous System Rats Sprague-Dawley Colon Sigmoid Laparotomy Internal medicine medicine Animals Hirschsprung Disease Intestinal Mucosa Intraoperative Complications Myenteric plexus business.industry Sigmoid colon General Medicine Abdominal distension medicine.disease Ganglion Rats Disease Models Animal medicine.anatomical_structure Pediatrics Perinatology and Child Health Surgery Enteric nervous system medicine.symptom business |
| Zdroj: | Pediatric surgery international. 30(8) |
| ISSN: | 1437-9813 |
| Popis: | A number of patients operated on for Hirschsprung disease continue to have constipation and abdominal distension for years after surgery. Some authors have proposed that ischemia during surgery may induce secondary aganglionosis. The aim of the present study was to study the effects of ischemia on the enteric nervous system of sigmoid colon in an animal model. A surgical model of colonic ischemia was created. 34 adult Sprague–Dawley rats underwent a laparotomy where the marginal arterioles of the sigmoid colon were ligated. After that, a section in the middle segment of the sigmoid colon was performed followed by an anastomosis. The presence of ischemia was assessed by measurement of visible light spectroscopy tissue oximetry and histological examination. Colonic function was assessed by evaluation of stool weight. Rats were killed at 1, 8 and 12 weeks after the operation. 12 rats were sham-operated. Enteric nervous system was evaluated by means of immunohistochemistry with NGFR p75. Quantitative analysis of the number of ganglia and ganglion cells in the myenteric plexus was performed. The surgical model of colonic ischemia significantly decreased tissue oxygenation (pre-surgical = 54.69 ± 7.32 %; post-surgical = 27.37 ± 9.2 %; p |
| Databáze: | OpenAIRE |
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