Monoclonal antibodies inhibitory to human plasmin. Definitive demonstration of a role for plasmin in activating the proenzyme of urokinase-type plasminogen activator
| Autor: | Pheng-Siew Sim, David R. H. Fayle, William F. Doe, Ross W. Stephens |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Plasmin
medicine.drug_class medicine.medical_treatment Monoclonal antibody Biochemistry Chromatography Affinity Plasminogen Activators Enzyme activator Affinity chromatography Fibrinolysis medicine Humans Fibrinolysin Urokinase Enzyme Precursors biology Chemistry Antibodies Monoclonal Plasminogen Urokinase-Type Plasminogen Activator Molecular biology Enzyme Activation Molecular Weight Colonic Neoplasms biology.protein Electrophoresis Polyacrylamide Gel Antibody Plasminogen activator medicine.drug |
| Zdroj: | European Journal of Biochemistry. 158:537-542 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1986.tb09787.x |
| Popis: | Four monoclonal antibodies raised against purified human plasminogen were characterized for their effects on the activation of plasminogen and on three enzymic properties of plasmin: (a) thioesterolysis, (b) fibrinolysis, (c) conversion of high-Mr urokinase to its low-Mr form. None of the monoclonal antibodies inhibited plasminogen (plg) activation by urokinase. The monoclonal antibodies characterized in this study fell into three groups. Anti-plg 1 inhibited (a), (b) and (c), while anti-plg 2 inhibited activities (a), (b) and (c) to varying degrees but also formed complexes with plasmin that were stable to sodium dodecyl sulphate. Anti-plg 3 and anti-plg 4 inhibited only activity (c). Selective use of these monoclonal antibodies demonstrated unequivocally that plasmin mediates the activation of the proenzyme form of urokinase-type plasminogen activator. Besides their use in affinity chromatography, therefore, these antibodies are valuable for defining the role of plasmin in the mechanisms of extracellular matrix degradation. |
| Databáze: | OpenAIRE |
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