Shenxiong Drop Pill exerts neuroprotective effect against focal cerebral ischemia partly via regulation of the expressions of ICAM-1 and caspase-3
| Autor: | Ning-jing Ran, Meng-yuan Huang, Wei-yin Chen, Meng Hou, Yang Zhang, Shuo-guo Jin, Fang Yang, Xin-xia Zhang, Ze-ran Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
ICAM-1
Cerebral infarction Chemistry Ischemia Pharmaceutical Science Inflammation Caspase 3 Pharmacology medicine.disease Neuroprotection Staining symbols.namesake Acute ischemic stroke Neuroprotection Inflammation Middle cerebral artery occlusion Caspase-3 Intercellular adhesion molecule 1 medicine Nissl body symbols Pharmacology (medical) medicine.symptom |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol. 20 No. 10 (2021); 2015-2022 |
| ISSN: | 1596-5996 1596-9827 |
| Popis: | Purpose: To investigate the effect of Shenxiong Drop Pill (SXDP) on cerebral infarction (CI) in rats, and the involvement of anti-inflammatory response in the process.Methods: Rats were sacrificed at three different time points, viz, 24, 48 and 72 h after establishment of CI model. Neurological deficit score (NDS) was determined using Bederson’s neurological behavioral scoring method, whereas triphenyltetrazolium chloride (TTC) staining was used to show brain injury. The integrated optical density (IOD) of Nissl bodies and caspase-3-positive nerve cells were measured with Nissl staining and SP kit, respectively. The mRNA expression of intercellular adhesion molecule 1(ICAM-1) was determined using reverse transcription-polymerase chain reaction (RT-PCR).Results: SXDP produced neuroprotective effect at high, medium, and low doses. The infarct volumes in the high-, medium- and low-dose SXDP, and cyclophosphamide groups were significantly reduced at each time point. Different doses of SXDP significantly reduced the mRNA expression of ICAM-1 and the IOD of caspase-3.Conclusion: These results indicate that SXDP exerts neuroprotective effects against ischemic injury by negatively regulating ICAM-1/caspase-3 downstream of inflammatory and apoptosis pathways. |
| Databáze: | OpenAIRE |
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