RNA interference targeting ecdysone receptor blocks the larval–pupal transition in Henosepilachna vigintioctopunctata
| Autor: | Ahmad Ali Anjum, Long-Ji Ze, Guo-Qing Li, Chen-Hui Shen, Jian-Jian Wu, Wei-Nan Kang, Lin Jin, Li-Li Mu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Receptors Steroid animal structures Henosepilachna vigintioctopunctata Environmental pollution Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences RNA interference Escherichia coli Animals Bioassay Ecology Evolution Behavior and Systematics RNA Double-Stranded fungi Pupa Pesticide biology.organism_classification Coleoptera 010602 entomology Horticulture RNA silencing 030104 developmental biology Larva Insect Science Insect Proteins RNA Interference PEST analysis Microorganisms Genetically-Modified Ecdysone receptor Agronomy and Crop Science |
| Zdroj: | Insect Science. 28:419-429 |
| ISSN: | 1744-7917 1672-9609 |
| DOI: | 10.1111/1744-7917.12777 |
| Popis: | Henosepilachna vigintioctopunctata is a serious insect pest which attacks a large number of nightshades and cucurbits in Asian countries, Brazil and Australia. Prolonged application of traditional pesticides has caused environmental pollution and exerted deleterious effects on human health. Finding new approaches with high target specificity and low environmental contamination has become an urgent task. RNA interference (RNAi) induced by double-stranded RNA (dsRNA) is expected to be applicable to managing this pest. Here we evaluated the effects of Escherichia coli-expressed dsRNAs targeting ecdysone receptor (EcR) gene via dietary delivery in laboratory and foliar spraying in a greenhouse. The target transcript was successfully knocked down when the 4th-instar larvae had fed on potato foliage dipped with dsEcR in a laboratory bioassay. Around 85% of the HvEcR RNAi larvae remained as prepupae or became abnormal pupae, and failed to emerge into adults. Ingestion of dsEcR-immersed foliage by the 3rd-instar larvae effectuated a comparable RNAi response and brought about more severe defects: all the resultant larvae arrested development, remained as prepupae and finally died. For assay in the greenhouse, a dsEcR-contained E. coli suspension was directly sprayed to the foliage of greenhouse-growing potato plants and the 3rd- and 4th-instar larvae were transferred to the leaves. High RNAi efficacy was obtained and identical RNAi phenotypes were observed in treated larvae. In addition, spraying dsEcR reduced leaf damage. Our results indicate a possibility of practical application of dsEcR as an environmentally friendly RNA pesticide to control H. vigintioctopunctata larvae. |
| Databáze: | OpenAIRE |
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