Handheld analyzer with on-chip molecularly-imprinted biosensors for electrical detection of propofol in plasma samples
| Autor: | Meng-Hua Chung, Pei-Wen Hsieh, Chih-Chung Lin, Chien-Chong Hong, Chian-Lang Hong, Zi-Xiang Lin |
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| Rok vydání: | 2016 |
| Předmět: |
Spectrum analyzer
Materials science Conductometry Ion-mobility spectrometry Microfluidics Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques 02 engineering and technology Sensitivity and Specificity 01 natural sciences Molecular Imprinting Lab-On-A-Chip Devices Electrochemistry medicine Disposable Equipment Biochip Propofol Detection limit Miniaturization 010401 analytical chemistry Molecularly imprinted polymer Reproducibility of Results Equipment Design General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Equipment Failure Analysis Point-of-Care Testing 0210 nano-technology Biosensor Biotechnology Biomedical engineering medicine.drug |
| Zdroj: | Biosensors and Bioelectronics. 86:623-629 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.07.032 |
| Popis: | This paper proposes a novel handheld analyzer with disposable lab-on-a-chip technology for the electrical detection of the anesthetic propofol in human plasma samples for clinical diagnoses. The developed on-chip biosensors are based on the conduction of molecularly imprinted polymers (MIPs) that employ label-free electrical detection techniques. Propofol in total intravenous anesthesia is widely used with a target-controlled infusion system. At present, the methods employed for detecting blood propofol concentrations in hospitals comprise high-performance liquid chromatography and ion mobility spectrometry. These conventional instruments are bulky, expensive, and difficult to access. In this study, we developed a novel plastic microfluidic biochip with an on-chip anesthetic biosensor that was characterized for the rapid detection of propofol concentrations. The experimental results revealed that the response time of the developed propofol biosensors was 25 s. The specific binding of an MIP to a nonimprinted polymer (NIP) reached up to 560%. Moreover, the detection limit of the biosensors was 0.1 μg/mL, with a linear detection range of 0.1–30 μg/mL. The proposed disposable microfluidic biochip with an on-chip anesthetic biosensor using MIPs exhibited excellent performance in the separation and sensing of propofol molecules in the human plasma samples. Compared with large-scale conventional instruments, the developed microfluidic biochips with on-chip MIP biosensors present the advantages of a compact size, high selectivity, low cost, rapid response, and single-step detection. |
| Databáze: | OpenAIRE |
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