Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells
| Autor: | Hung Sheng Shang, Shu Ching Hsueh, Jing Gung Chung, Hsiu Chen Chou, Jason Chou, Ming Yang Yeh, Yung Luen Shih, Fu Shin Chueh, Yung Lin Chu, Hsiao Min Chou, Hsu Feng Lu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research DNA Repair DNA repair DNA damage Poly ADP ribose polymerase Blotting Western Biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Animals Microscopy Phase-Contrast DAPI Melanoma Flavonoids Microscopy Confocal General Medicine Cell cycle Flow Cytometry Antineoplastic Agents Phytogenic Molecular biology MDC1 Disease Models Animal 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Cancer cell Casticin Cancer research DNA Damage |
| Zdroj: | Oncology Reports. 36:2094-2100 |
| ISSN: | 1791-2431 1021-335X |
| Popis: | Casticin, a polymethoxyflavone, has been demonstrated to possess anticancer activities, yet no study has shown in detail that casticin induces DNA damage in lung cancer cells. The purpose of this study was to investigate the possible molecular mechanisms of casticin which induce DNA damage and nuclear condensation in murine melanoma cancer B16F10 cells. In this study, by examining and capturing images using phase contrast microscopy, we found that casticin induced cell morphological changes. Moreover, it decreased the total number of viable cells which was measured by flow cytometry. Casticin-induced DNA damage and nuclear DNA condensation were measured by DAPI staining, respectively. Western blotting indicated that casticin decreased the protein levels of O6‑methylguanine-DNA methyltransferase (MGMT), breast cancer 1, early onset (BRCA1), mediator of DNA damage checkpoint 1 (MDC1), DNA-dependent protein kinase (DNA-PK) but increased phospho-p53 tumor suppressor protein (p-p53), phospho-ataxia telangiectasia mutated kinase (p-ATM), phospho-histone H2A.X (Ser139) and poly(ADP-ribose) polymerase (PARP) in the B16F10 cells. Furthermore, we used confocal laser system microscopy to examine the protein expression levels and we found that casticin increased the expression of p-p53 and p-H2A.X in the B16F10 cells. Collectively, casticin induced DNA damage and affected DNA repair proteins in the B16F10 cells in vitro. |
| Databáze: | OpenAIRE |
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