Empowering a mesophilic inoculum for thermophilic nitrification : growth mode and temperature pattern as critical proliferation factors for archaeal ammonia oxidizers
Autor: | Emilie Courtens, Marius Vital, Tom T. M. Vandekerckhove, Ken Meerbergen, Dietmar H. Pieper, Siegfried E. Vlaeminck, Bart Lievens, Ramiro Vilchez-Vargas, Delphine Prat, Nico Boon |
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Přispěvatelé: | Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany. |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Environmental Engineering Molecular Sequence Data Biology 03 medical and health sciences Nitrososphaera Bioreactors Ammonia RNA Ribosomal 16S Bioreactor Biomass Waste Management and Disposal Nitrites Phylogeny Water Science and Technology Civil and Structural Engineering Waste management Base Sequence Sewage Moving bed biofilm reactor Ecological Modeling Temperature Flocculation Nitrobacter biology.organism_classification Pulp and paper industry Pollution Archaea Nitrification Chemistry 030104 developmental biology Biodegradation Environmental Volatile suspended solids Biofilms Nitrospira Oxidation-Reduction Engineering sciences. Technology Mesophile |
Zdroj: | Water research |
ISSN: | 0043-1354 |
Popis: | Cost-efficient biological treatment of warm nitrogenous wastewaters requires the development of thermophilic nitrogen removal processes. Only one thermophilic nitrifying bioreactor was described so far, achieving 200 mg N L(-1) d(-1) after more than 300 days of enrichment from compost samples. From the practical point of view in which existing plants would be upgraded, however, a more time-efficient development strategy based on mesophilic nitrifying sludge is preferred. This study evaluated the adaptive capacities of mesophilic nitrifying sludge for two linear temperature increase patterns (non-oscillating vs. oscillating), two different slopes (0.25 vs. 0.08 °C d(-1)) and two different reactor types (floc vs. biofilm growth). The oscillating temperature pattern (0.25 °C d(-1)) and the moving bed biofilm reactor (0.08 °C d(-1)) could not reach nitrification at temperatures higher than 46 °C. However, nitrification rates up to 800 mg N L(-1) d(-1) and 150 mg N g(-1) volatile suspended solids d(-1) were achieved at a temperature as high as 49 °C by imposing the slowest linear temperature increase to floccular sludge. Microbial community analysis revealed that this successful transition was related with a shift in ammonium oxidizing archaea dominating ammonia oxidizing bacteria, while for nitrite oxidation Nitrospira spp. was constantly more abundant than Nitrobacter spp.. This observation was accompanied with an increase in observed sludge yield and a shift in maximal optimum temperature, determined with ex-situ temperature sensitivity measurements, predicting an upcoming reactor failure at higher temperature. Overall, this study achieved nitrification at 49 °C within 150 days by gradual adaptation of mesophilic sludge, and showed that ex-situ temperature sensitivity screening can be used to monitor and steer the transition process. |
Databáze: | OpenAIRE |
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