The γ3 Chain of Laminin is Widely But Differentially Expressed in Murine Basement Membranes: Expression and Functional Studies
| Autor: | William J. Brunken, Robert E. Burgeson, Gerard H. Daly, Stephanie Radner, Manuel Koch, Germán Pinzón-Duarte, Margaret M. French, Yong N. Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Central Nervous System
Heterozygote Cellular differentiation Longevity Morphogenesis Retinal Neovascularization Article Antibodies Basement Membrane Retina Mice Laminin Heterotrimeric G protein medicine Extracellular Animals Cloning Molecular Molecular Biology Alleles Basement membrane chemistry.chemical_classification Regulation of gene expression Mice Knockout Neurons biology Neovascularization Pathologic Integrin beta1 Molecular biology Immunohistochemistry Recombinant Proteins Mice Inbred C57BL medicine.anatomical_structure chemistry Gene Expression Regulation biology.protein Rabbits Glycoprotein |
| Popis: | Laminins are heterotrimeric extracellular glycoproteins found in, but not confined to, basement membranes (BMs). They are important components in formation of the molecular networks of BMs as well as in cell polarity, cell differentiation and tissue morphogenesis. Each laminin is composed by an α, a β and a γ chain. Previous studies have shown that the γ3 chain is partnered with either the β1 chain (in placenta) or β2 chain (in the CNS) (Libby et al., 2000). Several studies, including our own, suggested that the γ3 chain is expressed in both apical and basal compartments (Gersdorff et al., 2005; Koch et al., 1999; Yan and Cheng, 2006). This study investigates the expression pattern of the γ3 chain in mouse. We developed three new γ3-reactive antibodies, and we show that the γ3 chain is present in BMs. The distribution pattern is considerably more restricted than that of the γ1 chain and within any tissue there is differential deposition into BM compartments. This is particularly true in the retina and brain, where γ3 is uniquely expressed in a subset of the vascular basement membranes and the pial surface. We used conventional genetic ablation techniques to remove the γ3 chain in mice; unlike other laminin null mice (α5, β2, γ1 nulls) (Miner et al., 1998; Noakes et al., 1995; Smyth et al., 1999), these mice live a normal lifespan and have only minor abnormalities, the most striking of which are ectopic granule cells in the cerebellum and an apparent increase in capillary branching in the outer retina. These data support the suggestion that the γ3 chain is deposited in BMs and contributes some unique properties to their function, particularly in the nervous system. |
| Databáze: | OpenAIRE |
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