Preoperative Diagnosis of Suspicious Parathyroid Adenomas by RT-PCR Using mRNA Extracted from Leftover Cells in a Needle Used for Ultrasonically Guided Fine Needle Aspiration Cytology
| Autor: | Evelina Mendonça, J. M. Boavida, Sónia Pratas, Valeriano Leite, Luís G. Sobrinho, Branca M. Cavaco, Fernando Torrinha |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adenoma
Adult Male Pathology medicine.medical_specialty Histology Sensitivity and Specificity Pathology and Forensic Medicine Cytology Chromogranins medicine Humans RNA Messenger Aged Ultrasonography Parathyroid adenoma Hyperparathyroidism biology Reverse Transcriptase Polymerase Chain Reaction Parathyroid neoplasm business.industry Biopsy Needle Chromogranin A General Medicine Middle Aged medicine.disease Reverse transcription polymerase chain reaction Parathyroid Neoplasms Molecular Diagnostic Techniques Parathyroid Hormone Cytopathology biology.protein Female business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1938-2650 0001-5547 |
| DOI: | 10.1159/000326468 |
| Popis: | OBJECTIVE: To determine the usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) detection of parathormone (PTH) gene mRNA in needle aspirates to confirm the parathyroid nature of suspicious cervical lesions in patients with hyperparathyroidism. STUDY DESIGN: Ultrasound-guided fine needle aspiration cytology (FNAC) was performed on 12 patients with suspected parathyroid adenomas. The aspirates were subjected to cytologic and chromogranin A examination. Messenger RNA was isolated from leftover cells within the needle, and RT-PCR was used to amplify mRNA encoding PTH. RESULTS: The 12 aspirates were positive by PTH RT-PCR, and molecular diagnosis was confirmed by histologic examination. The lower sensitivity of cytologic and immunocytochemical methods was demonstrated, respectively, by 67% and 50% of positive results. Sensitivity assays demonstrated that with our system, PTH RT-PCR products are obtained from as few as 10 pg of parathyroid mRNA. CONCLUSION: The present study showed that RT-PCR-based analysis of PTH gene transcripts in aspirates, obtained by US-guided FNAC of cervical lesions suspicious for parathyroid adenoma, is a feasible, sensitive and specific method for preoperative diagnosis of parathyroid adenomas. |
| Databáze: | OpenAIRE |
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