Construction of a genetic sexing strain for Aedes albopictus: a promising tool for the development of sterilizing insect control strategies targeting the tiger mosquito
Autor: | Cyrille Lebon, Patrick Mavingui, Pablo Tortosa, Célestine M. Atyame, Aude Benlali |
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Přispěvatelé: | Cyclotron Réunion Océan Indien (CYROI), Université de La Réunion (UR)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), SymbioTIC, Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), International Atomic Energy, Coordinated Research Project (Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes), Credit Agricole, French Ministry of Research and higher education, Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Male
Mosquito Control Aedes albopictus 030231 tropical medicine Incompatible Insect Technique Zoology Mosquito Vectors Sexing Arbovirus Infections Biology lcsh:Infectious and parasitic diseases Animals Genetically Modified 03 medical and health sciences Sterile insect technique Dieldrin chemistry.chemical_compound [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems 0302 clinical medicine Aedes Animals Humans lcsh:RC109-216 sex separation Infertility Male 030304 developmental biology [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health 0303 health sciences Larva Research [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] fungi Sterile Insect Technique biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology 3. Good health [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics chemistry Vector (epidemiology) Female [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Arboviruses Sex ratio Sex linkage Genetic Sexing Strain |
Zdroj: | Parasites & Vectors Parasites & Vectors, 2018, 11 (S2), pp.658. ⟨10.1186/s13071-018-3212-y⟩ Parasites and Vectors Parasites and Vectors, BioMed Central, 2018, 11 (S2), pp.658. ⟨10.1186/s13071-018-3212-y⟩ Parasites and Vectors, BioMed Central, 2018, ⟨10.1186/s13071-018-3212-y⟩ Parasites & Vectors, Vol 11, Iss S2, Pp 57-64 (2018) |
ISSN: | 1756-3305 |
Popis: | BackgroundAedes albopictus is an invasive mosquito species of global medical concern as its distribution has recently expanded to Africa, the Americas and Europe. In the absence of prophylaxis protecting human populations from emerging arboviruses transmitted by this mosquito species, the most straightforward control measures rely on the suppression or manipulation of vector natural populations. A number of environmental-friendly methods using innundative releases of sterilizing males are currently under development. However, these strategies are still lacking an efficient sexing method required for mass production of males.ResultsWe present the first Genetic Sexing Strain (GSS) in Ae. albopictus, hereafter referred as TiCoq, obtained by sex linkage of rdl gene conferring dieldrin resistance. Hatching rate, larval survival and sex ratio were followed during twelve generations. The use of dieldrin at third larval stage allowed selecting 98% of males on average.ConclusionA good production rate of TiCoq males makes this GSS suitable for any control method based on mass production of Ae. albopictus sterilizing males. Despite limitations resulting from affected egg hatch as well as the nature of the used insecticide, the construction of this GSS paves the way for industrial sex separation of Ae. albopictus. |
Databáze: | OpenAIRE |
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