Construction of a genetic sexing strain for Aedes albopictus: a promising tool for the development of sterilizing insect control strategies targeting the tiger mosquito

Autor: Cyrille Lebon, Patrick Mavingui, Pablo Tortosa, Célestine M. Atyame, Aude Benlali
Přispěvatelé: Cyclotron Réunion Océan Indien (CYROI), Université de La Réunion (UR)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), SymbioTIC, Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), International Atomic Energy, Coordinated Research Project (Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes), Credit Agricole, French Ministry of Research and higher education, Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Mosquito Control
Aedes albopictus
030231 tropical medicine
Incompatible Insect Technique
Zoology
Mosquito Vectors
Sexing
Arbovirus Infections
Biology
lcsh:Infectious and parasitic diseases
Animals
Genetically Modified
03 medical and health sciences
Sterile insect technique
Dieldrin
chemistry.chemical_compound
[SDV.EE.ECO]Life Sciences [q-bio]/Ecology
environment/Ecosystems
0302 clinical medicine
Aedes
Animals
Humans
lcsh:RC109-216
sex separation
Infertility
Male
030304 developmental biology
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
0303 health sciences
Larva
Research
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
fungi
Sterile Insect Technique
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
chemistry
Vector (epidemiology)
Female
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Arboviruses
Sex ratio
Sex linkage
Genetic Sexing Strain
Zdroj: Parasites & Vectors
Parasites & Vectors, 2018, 11 (S2), pp.658. ⟨10.1186/s13071-018-3212-y⟩
Parasites and Vectors
Parasites and Vectors, BioMed Central, 2018, 11 (S2), pp.658. ⟨10.1186/s13071-018-3212-y⟩
Parasites and Vectors, BioMed Central, 2018, ⟨10.1186/s13071-018-3212-y⟩
Parasites & Vectors, Vol 11, Iss S2, Pp 57-64 (2018)
ISSN: 1756-3305
Popis: BackgroundAedes albopictus is an invasive mosquito species of global medical concern as its distribution has recently expanded to Africa, the Americas and Europe. In the absence of prophylaxis protecting human populations from emerging arboviruses transmitted by this mosquito species, the most straightforward control measures rely on the suppression or manipulation of vector natural populations. A number of environmental-friendly methods using innundative releases of sterilizing males are currently under development. However, these strategies are still lacking an efficient sexing method required for mass production of males.ResultsWe present the first Genetic Sexing Strain (GSS) in Ae. albopictus, hereafter referred as TiCoq, obtained by sex linkage of rdl gene conferring dieldrin resistance. Hatching rate, larval survival and sex ratio were followed during twelve generations. The use of dieldrin at third larval stage allowed selecting 98% of males on average.ConclusionA good production rate of TiCoq males makes this GSS suitable for any control method based on mass production of Ae. albopictus sterilizing males. Despite limitations resulting from affected egg hatch as well as the nature of the used insecticide, the construction of this GSS paves the way for industrial sex separation of Ae. albopictus.
Databáze: OpenAIRE
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