Total body water measurement using the 2H dilution technique for the assessment of body composition of Kuwaiti children
| Autor: | Badreya Al-Lahou, Fatima Al-Khulifi, Amani Al-Othman, Suad N. Al-Hooti, Jameela Al-Ghanim, Lena Davidsson, Tom Preston, Tareq Al-Ati, Nawal Al-Hamad |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pediatric Obesity medicine.medical_specialty Dilution technique Body water Indicator Dilution Techniques Medicine (miscellaneous) Urine Overweight Childhood obesity Body Mass Index Animal science Body Water Internal medicine Humans Medicine Child Adiposity Sex Characteristics Nutrition and Dietetics business.industry Public Health Environmental and Occupational Health Reproducibility of Results Deuterium medicine.disease Research Papers Total body water measurement Endocrinology Calibration Body Composition Female Composition (visual arts) medicine.symptom business Body mass index |
| Zdroj: | Public Health Nutr |
| ISSN: | 1475-2727 1368-9800 |
| Popis: | ObjectiveThe 2H dilution technique is the reference method to estimate total body water for body composition assessment. The aims of the present study were to establish the total body water technique at the Kuwait Institute for Scientific Research and assess body composition of Kuwaiti children.DesignThe isotope ratio mass spectrometer was calibrated with defined international reference water standards. A non-random sampling approach was used to recruit a convenience sample of Kuwaiti children. A dose of 2H2O, 1–3 g, was consumed after an overnight fast and 2H enrichment in baseline and post-dose urine samples was measured. Total body water was calculated and used to estimate fat-free mass. Fat mass was estimated as body weight minus fat-free mass.SettingThe total body water study was implemented in primary schools.SubjectsSeventy-five boys and eighty-three girls (7–9 years).ResultsMeasurements of the isotope ratio mass spectrometer were confirmed to be accurate and precise. Children were classified as normal weight, overweight or obese according to the WHO based on BMI-for-age Z-scores. Normal-weight and overweight girls had significantly higher percentage body fat (median (range): 32·4 % (24·7–39·3 %) and 38·3 % (29·3–44·2 %), respectively) compared with boys (median (range): 26·5 % (14·2–37·1 %) and 34·6 % (29·9–40·2 %), respectively). No gender difference was found in obese children (median 46·5 % v. 45·6 %).ConclusionsThe establishment of a state-of-the-art stable isotope laboratory for assessment of body composition provides an opportunity to explore a wide range of applications to better understand the relationship between body size, body composition and risk of developing non-communicable diseases in Kuwait. |
| Databáze: | OpenAIRE |
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