Establishment of 5′–3′ interactions in mRNA independent of a continuous ribose-phosphate backbone
| Autor: | Elmar Wahle, Michael Götze, Florian Kluge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RNA Caps
Untranslated region Polyadenylation RNA Stability Ribose poly(A) tail Biology Article Open Reading Frames 03 medical and health sciences Eukaryotic translation 5′–3′ interaction closed-loop mRNA decay translational repression Coding region RNA Messenger 3' Untranslated Regions Molecular Biology 030304 developmental biology 0303 health sciences Messenger RNA 030302 biochemistry & molecular biology RNA Translation (biology) Cell biology Open reading frame Eukaryotic Cells Protein Biosynthesis Ribosemonophosphates 5' Untranslated Regions Poly A |
| Zdroj: | RNA, 26 (5) RNA |
| ISSN: | 1355-8382 1469-9001 |
| DOI: | 10.3929/ethz-b-000412686 |
| Popis: | Functions of eukaryotic mRNAs are characterized by intramolecular interactions between their ends. We have addressed the question whether 5′ and 3′ ends meet by diffusion-controlled encounter “through solution” or by a mechanism involving the RNA backbone. For this purpose, we used a translation system derived from Drosophila embryos that displays two types of 5′–3′ interactions: Cap-dependent translation initiation is stimulated by the poly(A) tail and inhibited by Smaug recognition elements (SREs) in the 3′ UTR. Chimeric RNAs were made consisting of one RNA molecule carrying a luciferase coding sequence and a second molecule containing SREs and a poly(A) tail; the two were connected via a protein linker. The poly(A) tail stimulated translation of such chimeras even when disruption of the RNA backbone was combined with an inversion of the 5′–3′ polarity between the open reading frame and poly(A) segment. Stimulation by the poly(A) tail also decreased with increasing RNA length. Both observations suggest that contacts between the poly(A) tail and the 5′ end are established through solution, independently of the RNA backbone. In the same chimeric constructs, SRE-dependent inhibition of translation was also insensitive to disruption of the RNA backbone. Thus, tracking of the backbone is not involved in the repression of cap-dependent initiation. However, SRE-dependent repression was insensitive to mRNA length, suggesting that the contact between the SREs in the 3′ UTR and the 5′ end of the RNA might be established in a manner that differs from the contact between the poly(A) tail and the cap. RNA, 26 (5) ISSN:1355-8382 ISSN:1469-9001 |
| Databáze: | OpenAIRE |
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