Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
| Autor: | Paulo Roberto Slud Brofman, Lidiane Maria Boldrini Leite, Valderez Ravaglio Jamur, Luiz Guilherme Achcar Capriglione, Nelson Itiro Miyague, Marcia Olandoski, Gabriel Salles Ottoboni, Carmen Lúcia Kuniyoshi Rebelatto, Alexandra Cristina Senegaglia, Alejandro Correa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CD31 lcsh:Internal medicine Pathology medicine.medical_specialty Article Subject Population Cell Infarction 030204 cardiovascular system & hematology Umbilical cord Neovascularization 03 medical and health sciences 0302 clinical medicine medicine cardiovascular diseases Progenitor cell lcsh:RC31-1245 education Molecular Biology education.field_of_study business.industry Cell Biology medicine.disease Transplantation 030104 developmental biology medicine.anatomical_structure cardiovascular system medicine.symptom business Research Article |
| Zdroj: | Stem Cells International Stem Cells International, Vol 2018 (2018) |
| ISSN: | 1687-9678 1687-966X |
| Popis: | Pharmacological approaches are partially effective in limiting infarct size. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs) CD133+ have opened new perspectives for the treatment of ischemic areas after infarction. This preclinical study evaluated the effect of intramyocardial transplantation of purified or expanded human umbilical cord blood-derived CD133+ cells on the recovery of rats following acute myocardial infarction (AMI). Histology studies, electrocardiogram, and fluorescence in situ hybridization (FISH) were used to evaluate heart recovery. Purified CD133+ cells, enriched in endothelial progenitor cells, when expanded in vitro acquired an endothelial-like cell phenotype expressing CD31 and von Willebrand factor (vWF). The group of infarcted rats that received expanded CD133+ cells had a more significant recovery of contraction performance and less heart remodeling than the group that received purified CD133+ cells. Either purified or expanded CD133+ cells were able to induce neovascularization in the infarcted myocardium in an equivalent manner. Few human cells were detected in the infarcted myocardium of the rats 28 days after transplantation suggesting that the effects observed might be related primarily to paracrine activity. Although both cell populations ameliorated the infarcted heart and are suitable for regeneration of the vascular system, expanded CD133+ cells are more beneficial and promising candidates for vascular regeneration. |
| Databáze: | OpenAIRE |
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