Folic acid alleviates the blood brain barrier permeability and oxidative stress and prevents cognitive decline in sepsis-surviving rats
| Autor: | Aloir Neri de Oliveira Junior, Mariana Pereira de Souza Goldim, Richard Simon Machado, Jucélia Jeremias Fortunato, Kiuanne Lino Lobo Metzker, Naiana da Rosa, Everton Venícius Rosa Lanzzarin, Michele Novochadlo, Leandro Garbossa, Gabriela Costa Bernades, Sandra Bonfante, Khiany Mathias, Tatiana Barichello, Fabricia Petronilho, Larissa Joaquim, Jaqueline S. Generoso, Erick Bagio |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Perforation (oil well) 030204 cardiovascular system & hematology Blood–brain barrier medicine.disease_cause Biochemistry Antioxidants Lipid peroxidation Sepsis Capillary Permeability Protein Carbonylation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cognition Folic Acid Memory Internal medicine medicine TBARS Animals Cognitive Dysfunction Cognitive decline Rats Wistar biology Behavior Animal business.industry Cell Biology medicine.disease Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Blood-Brain Barrier Myeloperoxidase biology.protein Lipid Peroxidation Cardiology and Cardiovascular Medicine business Oxidative stress |
| Zdroj: | Microvascular research. 137 |
| ISSN: | 1095-9319 |
| Popis: | Sepsis is a complication of an infection which imbalance the normal regulation of several organ systems, including the central nervous system (CNS). Evidence points towards inflammation and oxidative stress as major steps associated with brain dysfunction in sepsis. Thus, we investigated the folic acid (FA) effect as an important antioxidant compound on acute brain dysfunction in rats and long term cognitive impairment and survival. Wistar rats were subjected to sepsis by cecal ligation and perforation (CLP) or sham (control) and treated orally with FA (10 mg/kg after CLP) or vehicle (veh). Animals were divided into sham + veh, sham + FA, CLP + veh and CLP + FA groups. Twenty-four hours after surgery, the hippocampus and prefrontal cortex were obtained and assayed for levels of blood brain barrier (BBB) permeability, nitrite/nitrate concentration, myeloperoxidase (MPO) activity, thiobarbituric acid reactive species (TBARS) formation and protein carbonyls. Survival was performed during 10 days after surgery and memory was evaluated. FA reduced BBB permeability, MPO activity in hippocampus and pre frontal cortex in 24 h and lipid peroxidation in hippocampus and improves the survival rate after sepsis. Long term cognitive improvement was verified with FA in septic rats compared with CLP + veh. Our data demonstrates that FA reduces the memory impairment in 10 days after sepsis and mortality in part by decreasing BBB permeability and oxidative stress parameters in the brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect