Production and secretion of interleukin-1alpha proteins by rat testis
| Autor: | Katarina Gustafsson, Arif Siddiqui, Cecilia K. Zetterström, Olle Söder, Günther Weber, Taranum Sultana, Brian P. Setchell |
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| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Male medicine.drug_class Biophysics Biology Biochemistry Rats Sprague-Dawley Paracrine signalling Mice Western blot Testis medicine Animals Secretion Receptor Molecular Biology Chromatography High Pressure Liquid medicine.diagnostic_test Chromatofocusing Cell Biology DNA Receptor antagonist Molecular biology Rats Molecular Weight Seminiferous tubule medicine.anatomical_structure Cell Division Interleukin-1 |
| Zdroj: | Biochemical and biophysical research communications. 297(3) |
| ISSN: | 0006-291X |
| Popis: | The present study characterizes constitutively expressed rat testicular interleukin-1alpha (IL-1alpha) proteins. IL-1 bioactivity of crude testis protein was completely neutralized by IL-1alpha antiserum, IL-1 receptor antagonist, and soluble type I IL-1 receptor. Upon non-denaturating gel permeation chromatography, bioactive IL-1 eluted at molecular sizes of 45, 31, and 17kDa and at charges of pH 5.7 and 6.0 after chromatofocusing. SDS-PAGE/Western blot analysis of proteins extracted from whole testis, seminiferous tubules, interstitial, and seminiferous tubule fluids all demonstrated IL-1alpha immunoreactivity at 45, 24, and 19kDa. Activated macrophages and tissue proteins from endotoxin treated rats showed immunoreactive 31 and 19kDa IL-1alpha. The results indicate that the testis produces three isoforms of IL-1alpha proteins that are secreted into the interstitial compartment and tubular lumen where they may exert paracrine functions. The testicular IL-1alpha isoforms may represent posttranslationally modified precursor, mature IL-1alpha, and a 24-kDa alternate splice form. |
| Databáze: | OpenAIRE |
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