Levels of Fecal Procyanidins and Changes in Microbiota and Metabolism in Mice Fed a High-Fat Diet Supplemented with Apple Peel
| Autor: | Wallace Yokoyama, Mendel Friedman, Carol Levin, Salem Elkahoui, Glenn E. Bartley |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Malus Adipose tissue Diet High-Fat High-performance liquid chromatography Catechin Feces Mice Sterol 14-Demethylase medicine Animals Biflavonoids Humans Proanthocyanidins Food science Obesity biology Bacteria food and beverages Polyphenols General Chemistry Metabolism biology.organism_classification Gastrointestinal Microbiome Polyphenol Fruit bacteria medicine.symptom General Agricultural and Biological Sciences Weight gain Stearoyl-CoA Desaturase |
| Zdroj: | Journal of agricultural and food chemistry. 67(37) |
| ISSN: | 1520-5118 |
| Popis: | The potential for apple peels to mitigate the deleterious effects of a high-fat diet in mice was investigated here. Mice were fed a high-fat diet supplemented with apple powders from three apple varieties or a commercial apple polyphenol. Polyphenols were characterized using colorimetric assays and high-performance liquid chromatography. Mice were tested for standard metabolic parameters. There was a dose response to dietary apple peels, with the higher intake leading to reduced weight gain and adipose tissue mass relative to the lower intake, but none of the treatments were statistically different from the control. The gene expression of liver enzyme stearoyl-CoA desaturase (Scd-1) was correlated with adipose weight, and liver enzyme cytochrome P51 (Cyp51) was downregulated by the apple diets. The feces from a subset of mice were analyzed for polyphenols and for bacteria taxa by next-generation sequencing. The results revealed that the makeup of the fecal microbiota was related to the metabolism of dietary polyphenols. |
| Databáze: | OpenAIRE |
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