An improved enzyme-linked immunosorbent assay of anti-collagen antibodies in human serum
| Autor: | Katsuyuki Fujii, Yutaka Nagai, Michiko Tsuji, Kuniaki Terato, Yasunori Shimozuru, Kagehisa Murota |
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| Rok vydání: | 1989 |
| Předmět: |
Tris
medicine.medical_specialty Hot Temperature Immunology Arthritis Enzyme-Linked Immunosorbent Assay Antibodies Immunoglobulin G Specimen Handling Arthritis Rheumatoid Microtiter plate chemistry.chemical_compound Antibody Specificity Internal medicine medicine Humans Immunology and Allergy chemistry.chemical_classification Chromatography biology Chemistry Hydrogen-Ion Concentration Serum samples medicine.disease Endocrinology Enzyme Rheumatoid arthritis biology.protein Collagen Antibody |
| Zdroj: | Journal of Immunological Methods. 124:63-70 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(89)90186-5 |
| Popis: | An improved enzyme-linked immunosorbent assay (ELISA) for the determination of anti-collagen antibodies in human serum has been developed. The method is based on the use of serum samples diluted to 1/50 with heat-inactivated normal rabbit serum adjusted to pH 8.0 with solid Tris (0.05 M), NaCl (0.15 M) and 2 M HCl. The use of normal rabbit serum minimizes non-specific adsorption of immunoglobulin G onto the plastic surface of microtiter plate. The applicability of the method for the quantitation of anti-collagen antibodies in human serum is demonstrated with 290 specimens of sera from normal controls (194) and patients with rheumatoid arthritis (96). |
| Databáze: | OpenAIRE |
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