The effects of nitrous oxide on a glutamate-gated ion channel and their reversal by high pressure; a single channel analysis
| Autor: | Alister Macdonald, R. L. Ramsey |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Kinetics Biophysics Glutamic Acid chemistry.chemical_element Grasshoppers Biochemistry Ion Channels 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Molar volume 030202 anesthesiology Hydrostatic Pressure Animals Anesthesia Helium Ion channel Nitrous oxide Muscles Gated Ion Channel Conductance Cell Biology equipment and supplies l-Glutamate High pressure Dwell time chemistry Female 030217 neurology & neurosurgery |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1236:135-141 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(95)00029-3 |
| Popis: | Nitrous oxide reversibly affects the kinetics, but not the conductance, of the gGIuR channel of locust muscle. 0.5 atm N 2 O at 20.5°C was without effect but both 1.5 and 2.7 atm significantly reduced the probability of the channel opening, the frequency of opening and the mean open time, and prolonged the mean closed time. 100 atm helium was without effect on these parameters, but when 98.5 atm He was combined with 1.5 atm N 2 O they, and the associated dwell time distributions, were restored to normal. 100 atm similarly combined with 2.7 atm N 2 O exerted a comparable trend which fell short of significance. The results are consistent with nitrous oxide binding to the channel with a significant molar volume increase, which pressure opposes. This suggests that nitrous oxide may cause conformational changes in the channel, and that the pressure reversal of nitrous oxide anaesthesia in animals could be caused by molecular antagonism. |
| Databáze: | OpenAIRE |
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