Matrix inhibition by Salmonella excludes uropathogenic E. coli from biofilm
| Autor: | C. S. Srinandan, Sandeep Miryala, Veena G Nair, S Chandramohan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Salmonella 030106 microbiology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Escherichia medicine Humans Uropathogenic Escherichia coli Shigella Escherichia coli Escherichia coli Infections Ecology biology Escherichia coli Proteins Biofilm Biofilm matrix Enterobacter biology.organism_classification Enterobacteriaceae 030104 developmental biology Biofilms |
| Zdroj: | FEMS Microbiology Ecology. 97 |
| ISSN: | 1574-6941 |
| Popis: | Biofilm is a predominant lifestyle of bacteria that comprises of cells as collectives enmeshed in a polymeric matrix. Biofilm formation is vital for bacterial species as it provides access to nutrients and protects the cells from environmental stresses. Here we show that interference in biofilm matrix production is a strategy by the competing bacterial species to reduce the ability of the other species to colonize a surface. Escherichia coli colonies that differ in matrix production display different morphologies on Congo red agar media, which we exploited for screening bacterial isolates capable of inhibiting the matrix. The cell-free supernatants from growth culture of the screened isolates impaired uropathogenic E. coli (UPEC) UTI89 strain's biofilm. A physicochemical analysis suggested that the compound could be a glycopeptide or a polysaccharide. Isolates that inhibited matrix production belonged to species of the family Enterobacteriaceae such as Shigella, Escherichia, Enterobacter and Salmonella. Competition experiments between the isolates and the UPEC strain resulted in mutual inhibition, particularly during biofilm formation causing significant reduction in productivity and fitness. Furthermore, we show that Salmonella strains competitively excluded the UPEC strain in the biofilm by inhibiting its matrix production, highlighting the role of interference competition. |
| Databáze: | OpenAIRE |
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