Identification of the Glucosyltransferase That Converts Hydroxymethyluracil to Base J in the Trypanosomatid Genome
| Autor: | Shuo Liu, Jessica Lopes da Rosa-Spiegler, Robert Sabatini, Whitney Bullard, Yinsheng Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Trypanosoma brucei brucei
Base J Protozoan Proteins Context (language use) macromolecular substances DNA and Chromosomes Biology medicine.disease_cause Biochemistry Genome Epigenesis Genetic Substrate Specificity Pentoxyl Gene Knockout Techniques chemistry.chemical_compound Glucosides parasitic diseases medicine RNA Messenger Uracil Molecular Biology Mutation fungi Cell Biology DNA Protozoan Thymine carbohydrates (lipids) chemistry Glucosyltransferases Mutagenesis Site-Directed biology.protein RNA Interference Glucosyltransferase Genome Protozoan RNA Protozoan DNA |
| Zdroj: | Journal of Biological Chemistry. 289:20273-20282 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m114.579821 |
| Popis: | O-linked glucosylation of thymine in DNA (base J) is an important regulatory epigenetic mark in trypanosomatids. β-d-glucopyranosyloxymethyluracil (base J) synthesis is initiated by the JBP1/2 enzymes that hydroxylate thymine, forming 5-hydroxymethyluracil (hmU). hmU is then glucosylated by a previously unknown glucosyltransferase. A recent computational screen identified a possible candidate for the base J-associated glucosyltransferase (JGT) in trypanosomatid genomes. We demonstrate that recombinant JGT utilizes uridine diphosphoglucose to transfer glucose to hmU in the context of dsDNA. Mutation of conserved residues typically involved in glucosyltransferase catalysis impairs DNA glucosylation in vitro. The deletion of both alleles of JGT from the genome of Trypanosoma brucei generates a cell line that completely lacks base J. Reintroduction of JGT in the JGT KO restores J synthesis. Ablation of JGT mRNA levels by RNAi leads to the sequential reduction in base J and increased levels of hmU that dissipate rapidly. The analysis of JGT function confirms the two-step J synthesis model and demonstrates that JGT is the only glucosyltransferase enzyme required for the second step of the pathway. Similar to the activity of the related Ten-Eleven Translocation (TET) family of dioxygenases on 5mC, our studies also suggest the ability of the base J-binding protein enzymes to catalyze iterative oxidation of thymine in trypanosome DNA. Here we discuss the regulation of hmU and base J formation in the trypanosome genome by JGT and base J-binding protein. |
| Databáze: | OpenAIRE |
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