Muscle memory: myonuclear accretion, maintenance, morphology, and miRNA levels with training and detraining in adult mice
| Autor: | Cory M. Dungan, Savannah R. Jones, Kaitlyn K. Frick, Kevin A. Murach, C. Brooks Mobley, John J. McCarthy, Christopher J. Zdunek, Charlotte A. Peterson |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Satellite Cells Skeletal Muscle Context (language use) Central nuclei Motor Activity miR‐1 Muscle hypertrophy 03 medical and health sciences Gastrocnemius muscle Mice 0302 clinical medicine Atrophy Muscle damage Physiology (medical) Internal medicine microRNA medicine Animals Orthopedics and Sports Medicine Muscle Skeletal Tet‐On business.industry Skeletal muscle Original Articles Hypertrophy H2B‐GFP medicine.disease musculoskeletal system Muscle memory (strength training) Mice Inbred C57BL MicroRNAs 030104 developmental biology medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis Female Original Article business human activities |
| Zdroj: | Journal of Cachexia, Sarcopenia and Muscle |
| ISSN: | 2190-6009 |
| Popis: | Background In the context of mass regulation, ‘muscle memory’ can be defined as long‐lasting cellular adaptations to hypertrophic exercise training that persist during detraining‐induced atrophy and may facilitate future adaptation. The cellular basis of muscle memory is not clearly defined but may be related to myonuclear number and/or epigenetic changes within muscle fibres. Methods Utilizing progressive weighted wheel running (PoWeR), a novel murine exercise training model, we explored myonuclear dynamics and skeletal muscle miRNA levels with training and detraining utilizing immunohistochemistry, single fibre myonuclear analysis, and quantitative analysis of miRNAs. We also used a genetically inducible mouse model of fluorescent myonuclear labelling to study myonuclear adaptations early during exercise. Results In the soleus, oxidative type 2a fibres were larger after 2 months of PoWeR (P = 0.02), but muscle fibre size and myonuclear number did not return to untrained levels after 6 months of detraining. Soleus type 1 fibres were not larger after PoWeR but had significantly more myonuclei, as well as central nuclei (P |
| Databáze: | OpenAIRE |
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