Micromechanical mass sensors for biomolecular detection in a physiological environment
| Autor: | Adriaan H. Bredekamp, Hans Peter Lang, Murali Krishna Ghatkesar, Thomas Braun, Christoph Gerber, Viola Barwich, Martin Hegner |
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| Rok vydání: | 2005 |
| Předmět: |
Immunoassay
Measurement method Materials science Cantilever business.industry Transducers Reproducibility of Results Nanotechnology Biosensing Techniques Equipment Design Sensitivity and Specificity Vibration Biomechanical Phenomena Equipment Failure Analysis Molecular Weight Adsorption Biopolymers Protein Interaction Mapping Optoelectronics Computer-Aided Design Sensitivity (control systems) Center frequency Silicon cantilever business |
| Zdroj: | Physical review. E, Statistical, nonlinear, and soft matter physics. 72(3 Pt 1) |
| ISSN: | 1539-3755 |
| Popis: | Received 1 April 2005; revised manuscript received 19 May 2005; published 14 September 2005Micromechanical cantilever arrays are used to measure time-resolved adsorption of tiny masses based onprotein-ligand interactions. Here, streptavidin-biotin interactions are investigated in a physiological environ-ment. A measurement method is introduced using higher flexural modes of a silicon cantilever in order toenhance the sensitivity of mass detection. Modeling the cantilever vibration in liquid allows the measurementof absolute mass changes. We show time-resolved mass adsorption of final 7±0.7 ng biotinylated latex beads.The sensitivity obtained is about 2.5 pg/Hz measuring at a center frequency of 750 kHz.DOI: 10.1103/PhysRevE.72.031907 PACS number s : 87.83. a, 68.47.Pe, 87.80. y, 46.40. f |
| Databáze: | OpenAIRE |
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