Interaction of lipopolysaccharide with detergents and its possible role in the detergent resistance of the outer membrane of gram-negative bacterua
| Autor: | Kathryn Nixdorff, Jobst Gmeiner, Hans Herbert Martin |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Mutant Biophysics Polysaccharide Biochemistry Permeability chemistry.chemical_compound Salmonella Proteus mirabilis chemistry.chemical_classification biology Vesicle Cell Membrane Polysaccharides Bacterial Biological Transport Drug Resistance Microbial Cell Biology biology.organism_classification Dextran Membrane chemistry Bacterial outer membrane Deoxycholic Acid |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 510:87-98 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(78)90132-3 |
| Popis: | In the presence of MgCl2, amounts of detergents which disrupted phospholipid vesicles caused lipopolysaccharide I from Proteus mirabilis to aggregate and form vesicular, membrane-like structures. Vesicle formation with P. mirabilis lipopolysaccharide II containing longer O-polysaccharide chains was extremely poor. Lipopolysaccharides of Salmonella minnesota R mutants (chemotypes Ra, Rc and Re) displayed a growing tendency for vesicle formation with increasing deficiency of the R core polysaccharide. Lipopolysaccharides of chemotypes Rc and Re produced vesicles even in the absence of MgCl2 and detergent. Spherical aggregates consisting of P. mirabilis lipopolysaccharide I, MgCl2 and detergent were unable to either entrap or retain [14C]-sucrose, [3H]inulin or [3H]dextran. On the other hand, S. minnesota R mutant lipopolysaccharides of chemotypes Rc and Re could entrap all three saccharides and retain them for at least short periods of time. Leakage of [3H]-inulin out of Re-lipopolysaccharide vesicles was greatly retarded by addition of MgCl2 to the vesicle system. Incorporation of P. mirabilis lipopolysaccharide I or S. minnesota Rc lipopolysaccharide into phospholipid vesicles protected these model membranes from disruption by detergent. This suggested a similar protective function of lipopolysaccharide in the outer membrane of enteric bacteria against the action of surfactants occuring in their normal intestinal habitat. |
| Databáze: | OpenAIRE |
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