Evidence of macrophage modulation in the mouse pubic symphysis remodeling during the end of first pregnancy and postpartum

Autor:	Kleber G. Franchini, P. S. L. de Oliveira, Jörg Kobarg, Sílvio Roberto Consonni, Daniel Martins-de-Souza, Paulo Pinto Joazeiro, Mariana Fioramonte, Marcelo Falsarella Carazzolle, Bianca Gazieri Castelucci, Ana Margarida Pereira
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Proteomics Cell biology Microarray Phagocytosis Immunology Reproductive biology lcsh:Medicine Pubic symphysis Biology Article Extracellular matrix Mice Pregnancy medicine Macrophage Animals Transcriptomics lcsh:Science Multidisciplinary Innate immune system Macrophages Postpartum Period lcsh:R Pubic Symphysis Immunity Innate Complement system Extracellular Matrix medicine.anatomical_structure Female lcsh:Q Bone Remodeling sense organs Homeostasis
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-68676-x
Popis:	In mouse pregnancy, pubic symphysis (PS) remodels into an elastic interpubic ligament (IpL) in a temporally regulated process to provide safe delivery. It restores at postpartum to assure reproductive tract homeostasis. Recently, macrophage localization in the IpL and dynamic changes in the expression of inflammatory mediators observed from the end of pregnancy (D18, D19) to early days postpartum (1dpp, 3dpp) highlighted the necessity of the identification of the key molecules involved in innate immune processes in PS remodeling. Therefore, this study uses morphological and high-sensitivity molecular techniques to identify both macrophage association with extracellular matrix (ECM) remodeling and the immunological processes involved in PS changes from D18 to 3dpp. Results showed macrophage association with active gelatinases and ECM components and 25 differentially expressed genes (DEGs) related to macrophage activities in interpubic tissues from D18 to 3dpp. Additionally, microarray and proteomic analysis showed a significant association of interpubic tissue DEGs with complement system activation and differentially expressed proteins (DEPs) with phagocytosis, highlighting the involvement of macrophage-related activities in mouse PS remodeling. Therefore, the findings suggest that PS ECM remodeling is associated with evidence of macrophage modulation that ensures both IpL relaxation and fast PS recovery postpartum for first labor.
Databáze:	OpenAIRE
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