Intratumoral Gold Nanoparticle-Enhanced CT Imaging: An in Vivo Investigation of Biodistribution and Retention
| Autor: | Sara Ricchetti, Alessandro Grattoni, Marc L. Sprouse, Dennis Wang, Nicola Ferrante, Matteo Hirsch, E. Brian Butler, Danilo Demarchi, Carly S. Filgueira, Rossana Terracciano |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Biodistribution biology Chemistry Computed tomography Tumors Attenuation In vivo Gold Economic indicators Nanoparticle Lewis lung carcinoma 02 engineering and technology 021001 nanoscience & nanotechnology Imaging phantom 03 medical and health sciences Colloidal gold biology.protein Distribution (pharmacology) Bovine serum albumin 0210 nano-technology 030304 developmental biology Biomedical engineering |
| Popis: | This study aims to evaluate the in vivo distribution of Gold Nanoparticles (GNPs) at different time points after intratumoral (IT) injection, exploiting their properties as contrast agents for Computed Tomography (CT). GNPs approximately 40 nm in diameter were synthesized with a surface plasmon peak at ~530 nm, capped with Bovine Serum Albumin (BSA) to improve colloidal stability, and characterized with standard methods. CT phantom imaging was performed to quantify X-ray attenuation as a function of GNP concentration and surface functionalization and to determine the appropriate particle dose for in vivo studies. Concentrated GNPs were intratumorally (IT) injected into Lewis Lung Carcinoma (LLC) solid tumors grown on the right flank of 6-week old female C57BL/6 mice. Ten days post-injection, follow up CT imaging was performed to assess the distribution and retention of the particles in the tumor. Using the CT attenuation quantification, images for each timepoint were segmented, and 3D volumes rendered, to conduct biodistribution analyses. The successful retention and permanence of the GNPs into the solid tumor after ten days suggests the significance of GNPs as a potential theranostic agent. |
| Databáze: | OpenAIRE |
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