Transcriptomic evidence that von Economo neurons are regionally specialized extratelencephalic-projecting excitatory neurons

Autor: Anna Marie Yanny, Madeline L. Berkowitz-Cerasano, Brian E. Kalmbach, Eliza Barkan, Songlin Ding, Richard H. Scheuermann, Kimberly A. Smith, Brian D. Aevermann, Danny N. Tran, John W. Phillips, Soraya I. Shehata, Amy Bernard, Rebecca D. Hodge, Christof Koch, Jamison McCorrison, Trygve E. Bakken, Jonathan T. Ting, Nicholas J. Schork, Susan M. Sunkin, Mark Novotny, Frank J. Steemers, Francisco Díez-Fuertes, Ed Lein, Pratap Venepally, Jeremy A. Miller, Charles Cobbs, Roger S. Lasken
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Telencephalon
Genetics of the nervous system
General Physics and Astronomy
Homology (biology)
Transcriptome
Mice
0302 clinical medicine
lcsh:Science
In Situ Hybridization
Fluorescence
In Situ Hybridization
Neurons
Temporal cortex
0303 health sciences
Multidisciplinary
Pyramidal Cells
General Neuroscience
Brain
Phenotype
Temporal Lobe
3. Good health
Electrophysiology
medicine.anatomical_structure
Neurological
Excitatory postsynaptic potential
Science
1.1 Normal biological development and functioning
In situ hybridization
Biology
Molecular neuroscience
General Biochemistry
Genetics and Molecular Biology
Article
Fluorescence
03 medical and health sciences
Underpinning research
Genetics
medicine
Animals
Humans
Patch clamp
Anterior cingulate cortex
030304 developmental biology
Gene Expression Profiling
Neurosciences
General Chemistry
Cellular neuroscience
Gene expression profiling
030104 developmental biology
nervous system
lcsh:Q
Neuron
Nucleus
Neuroscience
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature communications, vol 11, iss 1
Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-020-14952-3
Popis: von Economo neurons (VENs) are bipolar, spindle-shaped neurons restricted to layer 5 of human frontoinsula and anterior cingulate cortex that appear to be selectively vulnerable to neuropsychiatric and neurodegenerative diseases, although little is known about other VEN cellular phenotypes. Single nucleus RNA-sequencing of frontoinsula layer 5 identifies a transcriptomically-defined cell cluster that contained VENs, but also fork cells and a subset of pyramidal neurons. Cross-species alignment of this cell cluster with a well-annotated mouse classification shows strong homology to extratelencephalic (ET) excitatory neurons that project to subcerebral targets. This cluster also shows strong homology to a putative ET cluster in human temporal cortex, but with a strikingly specific regional signature. Together these results suggest that VENs are a regionally distinctive type of ET neuron. Additionally, we describe the first patch clamp recordings of VENs from neurosurgically-resected tissue that show distinctive intrinsic membrane properties relative to neighboring pyramidal neurons.
Little is known about von Economo neurons, which have been described in a subset of mammals and appear to be selectively lost in several human neurological diseases. Here, authors reveal the gene expression profile of these cells and show that they are likely long-distance projection neurons.
Databáze: OpenAIRE
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