The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection

Autor: Jean-Luc Davignon, Karima Naciri, Rabie Chelbi, Gilles Bessou, Chuang Dong, Nils Collinet, Michael Valente, Geoffray Brelurut, Pierre Milpied, Abdenour Abbas, Morgane Thomas-Chollier, Noudjoud Attaf, Thien-Phong Vu Manh, Inaki Cervera-Marzal, Alexandra-Chloé Villani, Benjamin Rauwel, Denis Thieffry, Marc Dalod, Elena Tomasello
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA, Partenaires INRAE, Center for Immunology and Inflammatory Diseases [Boston, MA, USA], Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), European Project: 281225,EC:FP7:ERC,ERC-2011-StG_20101109,SYSTEMSDENDRITIC(2012)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
CD4-Positive T-Lymphocytes
0301 basic medicine
MESH: CD4-Positive T-Lymphocytes / immunology
MESH: Signal Transduction
Muromegalovirus
[SDV]Life Sciences [q-bio]
Leukemia inhibitory factor receptor
Lymphocyte Activation
MESH: Mice
Knockout
Mice
0302 clinical medicine
Single-cell analysis
Interferon
MESH: Sequence Analysis
RNA
Immunology and Allergy
MESH: Microscopy
Confocal
MESH: Animals
MESH: Interferon Type I / metabolism
MESH: Tumor Necrosis Factor-alpha / metabolism
Cells
Cultured
ComputingMilieux_MISCELLANEOUS
Mice
Knockout
Mice
Inbred BALB C
Microscopy
Confocal
medicine.diagnostic_test
hemic and immune systems
Herpesviridae Infections
3. Good health
Cell biology
medicine.anatomical_structure
Plasmacytoid dendritic cells
Interferon Type I
MESH: Herpesviridae Infections / immunology
Tumor necrosis factor alpha
Single-Cell Analysis
MESH: Dendritic Cells / immunology
Signal Transduction
medicine.drug
MESH: Cells
Cultured
T cell
Immunology
MESH: Mice
Inbred BALB C
Biology
Article
Flow cytometry
03 medical and health sciences
Gene expression analysis
Downregulation and upregulation
In vivo
MESH: Mice
Inbred C57BL
medicine
Animals
MESH: Lymphocyte Activation
Sequence Analysis
RNA
Tumor Necrosis Factor-alpha
Dendritic Cells
Mice
Inbred C57BL
Confocal microscopy
030104 developmental biology
MESH: Muromegalovirus / physiology
030215 immunology
MESH: Single-Cell Analysis
Zdroj: Nature Immunology
Nature Immunology, Nature Publishing Group, 2020, 21 (9), pp.983-997. ⟨10.1038/s41590-020-0731-4⟩
Nature Immunology, 2020, 21 (9), pp.983-997. ⟨10.1038/s41590-020-0731-4⟩
Nature immunology
ISSN: 1529-2908
1529-2916
Popis: International audience; Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial, and more studies are needed to understand their orchestration. In the present study, we characterize in depth and link pDC activation states in animals infected by mouse cytomegalovirus by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but these occurred sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of leukemia inhibitory factor receptor and was promoted by cell-intrinsic tumor necrosis factor signaling. We propose a new model for how individual pDCs are endowed to exert different functions in vivo during a viral infection, in a manner tightly orchestrated in time and space.
Databáze: OpenAIRE
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