The activation trajectory of plasmacytoid dendritic cells in vivo during a viral infection
| Autor: | Jean-Luc Davignon, Karima Naciri, Rabie Chelbi, Gilles Bessou, Chuang Dong, Nils Collinet, Michael Valente, Geoffray Brelurut, Pierre Milpied, Abdenour Abbas, Morgane Thomas-Chollier, Noudjoud Attaf, Thien-Phong Vu Manh, Inaki Cervera-Marzal, Alexandra-Chloé Villani, Benjamin Rauwel, Denis Thieffry, Marc Dalod, Elena Tomasello |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA, Partenaires INRAE, Center for Immunology and Inflammatory Diseases [Boston, MA, USA], Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), European Project: 281225,EC:FP7:ERC,ERC-2011-StG_20101109,SYSTEMSDENDRITIC(2012) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine MESH: CD4-Positive T-Lymphocytes / immunology MESH: Signal Transduction Muromegalovirus [SDV]Life Sciences [q-bio] Leukemia inhibitory factor receptor Lymphocyte Activation MESH: Mice Knockout Mice 0302 clinical medicine Single-cell analysis Interferon MESH: Sequence Analysis RNA Immunology and Allergy MESH: Microscopy Confocal MESH: Animals MESH: Interferon Type I / metabolism MESH: Tumor Necrosis Factor-alpha / metabolism Cells Cultured ComputingMilieux_MISCELLANEOUS Mice Knockout Mice Inbred BALB C Microscopy Confocal medicine.diagnostic_test hemic and immune systems Herpesviridae Infections 3. Good health Cell biology medicine.anatomical_structure Plasmacytoid dendritic cells Interferon Type I MESH: Herpesviridae Infections / immunology Tumor necrosis factor alpha Single-Cell Analysis MESH: Dendritic Cells / immunology Signal Transduction medicine.drug MESH: Cells Cultured T cell Immunology MESH: Mice Inbred BALB C Biology Article Flow cytometry 03 medical and health sciences Gene expression analysis Downregulation and upregulation In vivo MESH: Mice Inbred C57BL medicine Animals MESH: Lymphocyte Activation Sequence Analysis RNA Tumor Necrosis Factor-alpha Dendritic Cells Mice Inbred C57BL Confocal microscopy 030104 developmental biology MESH: Muromegalovirus / physiology 030215 immunology MESH: Single-Cell Analysis |
| Zdroj: | Nature Immunology Nature Immunology, Nature Publishing Group, 2020, 21 (9), pp.983-997. ⟨10.1038/s41590-020-0731-4⟩ Nature Immunology, 2020, 21 (9), pp.983-997. ⟨10.1038/s41590-020-0731-4⟩ Nature immunology |
| ISSN: | 1529-2908 1529-2916 |
| DOI: | 10.1038/s41590-020-0731-4⟩ |
| Popis: | International audience; Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN-I). What other functions pDCs exert in vivo during viral infections is controversial, and more studies are needed to understand their orchestration. In the present study, we characterize in depth and link pDC activation states in animals infected by mouse cytomegalovirus by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal microscopy and a cognate CD4 T cell activation assay. We show that IFN-I production and T cell activation were performed by the same pDC, but these occurred sequentially in time and in different micro-anatomical locations. In addition, we show that pDC commitment to IFN-I production was marked early on by their downregulation of leukemia inhibitory factor receptor and was promoted by cell-intrinsic tumor necrosis factor signaling. We propose a new model for how individual pDCs are endowed to exert different functions in vivo during a viral infection, in a manner tightly orchestrated in time and space. |
| Databáze: | OpenAIRE |
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