Antibacterial and cytotoxic evaluation of copper and zinc oxide nanoparticles as a potential disinfectant material of connections in implant provisional abutments: An in-vitro study
| Autor: | Pamela Ehrenfeld, Maria Francisca Pavicic, Helia Bello-Toledo, Diego Vergara-Llanos, Manuel Melendrez-Castro, Gabriela Sánchez-Sanhueza, A. F. Jaramillo, Andrés Díaz-Gómez, Tania Koning |
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| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
0301 basic medicine Disinfectant education Gingiva chemistry.chemical_element Microbial Sensitivity Tests Zinc 03 medical and health sciences 0302 clinical medicine Humans Cytotoxicity General Dentistry Cells Cultured health care economics and organizations Dental Implants biology technology industry and agriculture 030206 dentistry Cell Biology General Medicine Fibroblasts respiratory system biology.organism_classification Molecular biology Anti-Bacterial Agents 030104 developmental biology Otorhinolaryngology chemistry Apoptosis Cell culture Nanoparticles Zinc Oxide Antibacterial activity Copper Intracellular Bacteria Disinfectants |
| Zdroj: | Archives of Oral Biology. 122:105031 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2020.105031 |
| Popis: | Objective This study evaluates the antibacterial activity against mono and multispecies bacterial models and the cytotoxic effects of zinc oxide and copper nanoparticles(ZnO-NPs/Cu-NPs) in cell cultures of human gingival fibroblasts(HGFs). Design The antibacterial activities of ZnO-NPs and Cu-NPs against 4 bacteria species were tested according to their minimum inhibitory concentrations(MICs) and against mature multispecies anaerobic model by spectral confocal laser scanning microscopy. The viabilities and cytotoxic effects of ZnO-NPs and Cu-NPs to HGFs cell cultures were tested by MTT, LDH assays, production of ROS, and the activation of caspase-3. The results were analyzed using one-way ANOVA followed by Tukey tests, considering p Results For all strains, MICs of ZnO-NPs and Cu-NPs were in the range of 78.3 μg/mL-3906 μg/mL and 125 μg/mL-625 ug/mL, respectively. In a multispecies model, a significant decrease in the total biomass volume(μ3) was observed in response to exposure to 125 μg/mL of each NPs for which there was bactericidal activity. Significant differences were found between the volumes of viable and nonviable biomass exposed to nanostructures with Cu-NPs compared to ZnO-NPs. Both NPs induced mitochondrial dose-dependent cytotoxicity, ZnO-NPs increases LDH release and intracellular ROS generation. Cu-NPs at a concentration of 50 μg/mL induced production of cleaved caspase-3, activating the apoptotic pathway early and at low doses. Conclusions After 24 h, ZnO-NPs are biocompatible between 78−100 μg/mL and Cu-NPs below 50 μg/mL. Antibacterial activity in a monospecies model is strain dependent, and in a multispecies model was a lower doses after 10 min of exposure. |
| Databáze: | OpenAIRE |
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