The dorsal raphe nucleus in schizophrenia: a post mortem study of 5-hydroxytryptamine neurones
| Autor: | Margaret M. Esiri, T H Priddle, S. J. Cooper, Timothy J. Crow, R M Craven |
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| Rok vydání: | 2005 |
| Předmět: |
Decussation
Adult Male medicine.medical_specialty Psychosis Serotonin Histology Time Factors Tissue Fixation Pathology and Forensic Medicine Dorsal raphe nucleus Physiology (medical) Internal medicine Formaldehyde Medicine Humans Post-mortem interval Aged Trigeminal nerve Aged 80 and over Neurons business.industry Anatomy Middle Aged medicine.disease Immunohistochemistry Endocrinology Neurology Schizophrenia Raphe Nuclei Female Neurology (clinical) Brainstem Autopsy medicine.symptom Raphe nuclei business Artifacts |
| Zdroj: | Neuropathology and applied neurobiology. 31(3) |
| ISSN: | 0305-1846 |
| Popis: | The 5-hydroxytryptamine (5-HT, serotonin) system has been implicated in the pathophysiology and treatment of schizophrenia. In this study, we addressed the hypothesis that a deficit of 5-HT neurones, either inherited or acquired, is central to the developmental pathology of the disorder. We examined putative 5-HT neurones of the dorsal raphe nucleus (DRN) in post mortem, formalin-fixed tissue from 15 schizophrenic patients and 20 control subjects matched for age and gender. No significant difference was detected between these groups in the number or size (cross-sectional area or diameter) of tryptophan-hydroxylase-immunoreactive cell profiles viewed in transverse sections collected from the level of the trochlear decussation to the emergence of the trigeminal nerve. Profile number was not affected by age, gender, side of the brainstem (left or right) or post mortem interval; however, time in formalin correlated negatively with the number of neurones counted. Moreover, a significant negative correlation was detected between time in formalin and the levels of immunoreaction product (optical density), which in turn correlated positively with our profile counts. A positive correlation was found between the age of subjects and our estimates of cell size. Our results do not support the proposal that an abnormality in the number and/or size of DRN 5-HT neurones is central to the aetiopathology of schizophrenia. |
| Databáze: | OpenAIRE |
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