Identification of a novel tumor transforming gene GAEC1 at 7q22 which encodes a nuclear protein and is frequently amplified and overexpressed in esophageal squamous cell carcinoma
| Autor: | R. W.L. Cheung, John Wong, Sai Wah Tsao, C. H. Chui, Yun Wen Chen, F. B.F. Law, Simon Law, Kai-Yau Wong, Qian Tao, Johnny Cheuk On Tang, King Y. Lam, Ping Yin Lee, Gopesh Srivastava, J. Ying, Cordelia Langford |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Esophageal Neoplasms Molecular Sequence Data Mice Nude Biology medicine.disease_cause Article Mice Cell Line Tumor Gene duplication Genetics medicine Animals Humans Amino Acid Sequence Nuclear protein Molecular Biology Gene Peptide sequence Cell Nucleus Base Sequence Models Genetic Esophageal disease Nuclear Proteins Esophageal cancer medicine.disease Gene Expression Regulation Neoplastic Cell nucleus medicine.anatomical_structure Carcinoma Squamous Cell Cancer research Carcinogenesis Chromosomes Human Pair 7 Neoplasm Transplantation |
| Zdroj: | Oncogene. 26:5877-5888 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1210390 |
| Popis: | By comparative DNA fingerprinting, we identified a 357-bp DNA fragment frequently amplified in esophageal squamous cell carcinomas (ESCC). This fragment overlaps with an expressed sequence tag mapped to 7q22. Further 5' and 3'-rapid amplification of cDNA ends revealed that it is part of a novel, single-exon gene with full-length mRNA of 2052 bp and encodes a nuclear protein of 109 amino acids ( approximately 15 kDa). This gene, designated as gene amplified in esophageal cancer 1 (GAEC1), was located within a 1-2 Mb amplicon at 7q22.1 identified by high-resolution 1 Mb array-comparative genomic hybridization in 6/10 ESCC cell lines. GAEC1 was ubiquitously expressed in normal tissues including esophageal and gastrointestinal organs; with amplification and overexpression in 6/10 (60%) ESCC cell lines and 34/99 (34%) primary tumors. Overexpression of GAEC1 in 3T3 mouse fibroblasts caused foci formation and colony formation in soft agar, comparable to H-ras and injection of GAEC1-transfected 3T3 cells into athymic nude mice formed undifferentiated sarcoma in vivo, indicating that GAEC1 is a transforming oncogene. Although no significant correlation was observed between GAEC1 amplification and clinicopathological parameters and prognosis, our study demonstrated that overexpressed GAEC1 has tumorigenic potential and suggest that overexpressed GAEC1 may play an important role in ESCC pathogenesis. |
| Databáze: | OpenAIRE |
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