Induction of Cr(VI) reduction activity in an Anoxybacillus strain under heat stress: a biochemical and proteomic study
| Autor: | Debasish Bhattacharyya, Bijay Bal, Sangita Dutta, Sujoy K. Das Gupta, Subrata Pal, Sangita Chowdhury Paul, Jyotirmoy Mitra, Preeti Jain, Pamela Bhattacharya |
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| Rok vydání: | 2011 |
| Předmět: |
Chromium
DNA Bacterial Hot Temperature Proteome Molecular Sequence Data Anoxybacillus India Biology Microbiology DNA Ribosomal Hot Springs Bacterial Proteins Oxidoreductase Stress Physiological RNA Ribosomal 16S Chromate reductase activity Genetics Cluster Analysis Electrophoresis Gel Two-Dimensional Molecular Biology Phylogeny chemistry.chemical_classification Two-dimensional gel electrophoresis Strain (chemistry) Gene Expression Profiling Metabolism Sequence Analysis DNA Molecular biology Amino acid Biochemistry chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Oxidoreductases Oxidation-Reduction Mesophile |
| Zdroj: | FEMS microbiology letters. 331(1) |
| ISSN: | 1574-6968 |
| Popis: | A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37 °C. However, growing cells, cell suspensions, and cell-free extracts from 65 °C cultures showed higher Cr(VI) reduction activities when assayed at either 37 or 65 °C than those obtained from 37 °C cultures. On fractionation of extracts from cells grown at 65 °C, the chromate reductase activity assayed at 65 °C was found mostly in the soluble fraction. When log-phase cells growing at 37 °C were shifted to 65 °C, the stressed cells produced larger quantities of reactive oxygen species. Consequently, growth of the cells was retarded, but specific Cr(VI) reduction activity increased. 2D gel electrophoresis followed by MALDI-TOF MS/MS identified the proteins whose expression level changed as a result of heat stress. The upregulated set included proteins involved in cellular metabolism of sugar, nucleotide, amino acids, lipids and vitamins, oxidoreductase activity, and protein folding. The downregulated proteins are also involved in cellular metabolism, DNA binding, and environmental signal processing. |
| Databáze: | OpenAIRE |
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