Use of poly(I:C) stabilized with chitosan as a vaccine-adjuvant against viral hemorrhagic septicemia virus infection in zebrafish
| Autor: | Lilia S. Ulanova, Martin Speth, Tor Gjøen, Arturas Kavaliauskis, Øystein Evensen, Gareth Griffiths, S. Dios, Sung-Hyun Kim, Marianne Arnemo, Beatriz Novoa |
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| Přispěvatelé: | Research Council of Norway, Ministerio de Economía y Competitividad (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_treatment
Polynucleotides Biology Virus Microbiology Novirhabdovirus Adjuvants Immunologic Hemorrhagic Septicemia Viral medicine Animals Receptor Cells Cultured Zebrafish Chitosan Viral Vaccine Viral Vaccines Head Kidney biology.organism_classification Virology In vitro Poly I-C biology.protein Animal Science and Zoology Viral hemorrhagic septicemia Antibody Adjuvant Developmental Biology |
| Popis: | 11 pages, 1 table, 8 figures There is an urgent need for more efficient viral vaccines in finfish aquaculture worldwide. Here, we report the use of poly(I:C) stabilized with chitosan as an adjuvant for development of better finfish vaccines. The adjuvant was co-injected with inactivated viral hemorrhagic septicemia virus (VHSV) (CSpIC+iV vaccine) in adult zebrafish and its efficiency in protection against VHSV infection was compared to a live, attenuated VHS virus vaccine (aV). Both free and stabilized poly(I:C) were strong inducers of an antiviral state, measured by transcriptional activation of the genes of viral sensors: toll-like receptors, interferons, and interferon-stimulated genes, such as MXa within 48 h after injection. Both the CSpIC+iV and the aV formulations provided a significant protection against VHSV-induced mortality. However, when plasma from survivors was tested for neutralizing antibodies in an in vitro protection assay, we could not demonstrate any protective effect. On the contrary, plasma from aV vaccinated fish enhanced cytopathic effects, indicating that antibody-dependent entry may play a role in this system. Our results show that poly(I:C) is a promising candidate as an adjuvant for fish vaccination against viral pathogens, and that the zebrafish is a promising model for aquaculture-relevant vaccination studies. This study was funded by grant no. 190625 from MLS@UiO to A.K. and T.G. and by the Jeju Flounder Cluster, South Korea, and partly from the Research Council of Norway, project no. 199813 “VHS virus–elucidation of pathogenic mechanisms” and project no. 225293 “The importance of the UTR panhandle structure of VHS virus for replication and virulence” to Ø.E. S.D. and B.N. were also supported by AGL2011-28921-C03 and AGL2014-51773-C3-2 from Ministerio de Economía and Competitividad, Spain. |
| Databáze: | OpenAIRE |
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