Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil
| Autor: | Samantha Serra Costa, Bruna Aparecida Souza Machado, Gabriele de Abreu Barreto, Francine Ferreira Padilha, Hugo Neves Brandão, Danielle Figuerêdo da Silva, Odir Antônio Dellagostin, João Antonio Pêgas Henriques, Rejane Pina Dantas Silva, Marcelo Andrés Umsza-Guez, José Luiz Carneiro da Rocha |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Antioxidant Coumaric Acids DPPH medicine.medical_treatment lcsh:Medicine Antioxidants Propolis Mice 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Anti-Infective Agents Phenols Cell Line Tumor Botany Escherichia coli medicine Animals Food science lcsh:Science Chromatography High Pressure Liquid Cell Proliferation Flavonoids Multidisciplinary ABTS Ethanol Phenylpropionates lcsh:R Extraction (chemistry) Supercritical fluid extraction Chromatography Supercritical Fluid Biological activity 04 agricultural and veterinary sciences Antineoplastic Agents Phytogenic 040401 food science 030104 developmental biology chemistry Microscopy Electron Scanning lcsh:Q Propionates Brazil Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 1, p e0145954 (2016) |
| ISSN: | 1932-6203 |
| Popis: | The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection. |
| Databáze: | OpenAIRE |
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